Construct: ORF TRCN0000477245
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000976.1_s317c1
- Derived from:
- ccsbBroadEn_01520
- DNA Barcode:
- ACTTTGCTCGTTGTCCGTAGAATT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SFRP1 (6422)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477245
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6422 | SFRP1 | secreted frizzled related p... | NM_003012.5 | 100% | 100% | |
| 2 | mouse | 20377 | Sfrp1 | secreted frizzled-related p... | NM_013834.3 | 92.4% | 94.9% | (many diffs) |
| 3 | mouse | 20377 | Sfrp1 | secreted frizzled-related p... | XM_011242128.1 | 92.4% | 94.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1008
- ORF length:
- 942
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg catcgggcgc agcgaggggg gccgccgcgg ggcagccctg ggcgtgctgc 121 tggcgctggg cgcggcgctt ctggccgtgg gctcggccag cgagtacgac tacgtgagct 181 tccagtcgga catcggcccg taccagagcg ggcgcttcta caccaagcca cctcagtgcg 241 tggacatccc cgcggacctg cggctgtgcc acaacgtggg ctacaagaag atggtgctgc 301 ccaacctgct ggagcacgag accatggcgg aggtgaagca gcaggccagc agctgggtgc 361 ccctgctcaa caagaactgc cacgccggca cccaggtctt cctctgctcg ctcttcgcgc 421 ccgtctgcct ggaccggccc atctacccgt gtcgctggct ctgcgaggcc gtgcgcgact 481 cgtgcgagcc ggtcatgcag ttcttcggct tctactggcc cgagatgctt aagtgtgaca 541 agttccccga gggggacgtc tgcatcgcca tgacgccgcc caatgccacc gaagcctcca 601 agccccaagg cacaacggtg tgtcctccct gtgacaacga gttGAAATCT GAGGCCATCA 661 TTGAACATCT CTGTGCCAGC GAGTTTGCAC TGAGGATGAA AATAAAAGAA GTGAAAAAAG 721 AAAATGGCGA CAAGAAGATT GTCCCCAAGA AGAAGAAGCC CCTGAAGTTG GGGCCCATCA 781 AGAAGAAGGA CCTGAAGAAG CTTGTGCTGT ACCTGAAGAA TGGGGCTGAC TGTCCCTGCC 841 ACCAGCTGGA CAACCTCAGC CACCACTTCC TCATCATGGG CCGCAAGGTG AAGAGCCAGT 901 ACTTGCTGAC GGCCATCCAC AAGTGGGACA AGAAAAACAA GGAGTTCAAA AACTTCATGA 961 AGAAAATGAA AAACCATGAG TGCCCCACCT TTCAGTCCGT GTTTAAGTGC CCAACTTTCT 1021 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 1081 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1141 GTGGAAAGGA CGAACTTTGC TCGTTGTCCG TAGAATTACG CGTTAAGTCg acaatcaacc 1201 tctggattac aaaatttgtg aaagatt