Construct: ORF TRCN0000477248
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017489.1_s317c1
- Derived from:
- ccsbBroadEn_10610
- DNA Barcode:
- GTCCTAATACATTCGCAAGGCCTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- BASP1-AS1 (285696)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477248
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 285696 | BASP1-AS1 | BASP1 antisense RNA 1 | NR_027253.1 | 17% | 1_448del;1016_3316del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 636
- ORF length:
- 567
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gacaccccct ggcaccagct acccttctca gaccccagtc cagcccgctc 121 ccgacgtcga ctacgattcc gctacctcgg ctggcagcga ggttggggtg agccccagct 181 gcaggcgcgt ctgggctgcg ccgctgcaaa cgagttgcgc accttgggcg gctccgcacc 241 tgcacccgca cccgcggggc tcagccccga aggctgcagc ttcgggggag gcgcggtcgc 301 cgaggtccag ctggtggggc gagagacgtc gccccTCGGA GGATGCTCTC GGAACTTGGG 361 AGAGGAAGGA GGGAAGAGAA GAGGGGAAAG GGGCCGTCGA TGTTTTTGAT GTCTGTGCTT 421 TAATGGAGGC CACCAATATT GAGAAGACGG GGTTGGCCGA GGCAGCCCGC ACGCTGCTGC 481 TTGCGAGCGC TCGAGTCAAA GCTAGGGCCA ACCGCGGCTT GTCCGGGTGC CCTAAGGGGG 541 CGGACACTTG GTTTAGCACC GGGACACAGA ATAGCCACCG GGGTAGGAAG ATGCGTTCAC 601 TTTGCTTACC TGTTGGCAAG AGGGACATAC AAAAATTGCC AACTTTCTTG TACAAAGTGG 661 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 721 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 781 AGTCCTAATA CATTCGCAAG GCCTTACGCG TTAAGTCgac aatcaacctc tggattacaa 841 aatttgtgaa agatt