Construct: ORF TRCN0000477257
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012046.1_s317c1
- Derived from:
- ccsbBroadEn_00664
- DNA Barcode:
- ATTTACCAATGCCCGATTCTAAAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GNS (2799)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477257
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2799 | GNS | glucosamine (N-acetyl)-6-su... | NM_002076.4 | 100% | 100% | |
| 2 | mouse | 75612 | Gns | glucosamine (N-acetyl)-6-su... | NM_029364.3 | 83.6% | 90.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1722
- ORF length:
- 1656
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcg gctcctgcct ctagccccag gtcggctccg gcggggcagc ccccgccacc 121 tgccctcctg cagcccagcg ctgctactgc tggtgctggg cggctgcctg ggggtcttcg 181 gggtggctgc gggaacccgg aggcccaacg tggtgctgct cctcacggac gaccaggacg 241 aagtgctcgg cggcatgaca ccgctaaaga aaaccaaagc tctcatcgga gagatgggga 301 tgactttttc cagtgcttat gtgccaagtg ctctctgctg ccccagcaga gccagtatcc 361 tgacaggaaa gtacccacat aatcatcacg ttgtgaacaa cactctggag gggaactgca 421 gtagtaagtc ctggcagaag atccaagaac caaatacttt cccagcaatt ctcagatcaa 481 tgtgtggtta tcagaccttt tttgcaggga aatatttaaa tgagtacgga gccccagatg 541 caggtggact agaacacgtt cctctgggtt ggagttactg gtatgccttg gaaaagaatt 601 ctaagtatta taattacacc ctgtctatca atgggaaggc acggaagcat ggtgaaaact 661 atagtgtgga ctacctgaca gatgttttgg ctaatgtctc cttggacttt ctggactaca 721 agtccaactt tgagcccttc ttcatgatga tcgccactcc agcgcctcat tcgccttgga 781 cagctgcacc tcagtaccag aaggctttcc agaatgtctt tgcaccaaga aacaagaact 841 tcaacatcca tggaacgaac aagcactggt taattaggca agccaagact ccaatgacta 901 attcttcaat acagttttta gataatgcat ttaggaaaag gtggcaaact ctcctctcag 961 ttgatgacct tgtggagaaa ctggtcaaga ggctggagtt cactggggag ctcaacaaca 1021 cttacatctt ctatacctca gacaatggct atcacacagg acagttttcc ttgccaatag 1081 acaagagaca gctgtatgag tttgatatca aagttccact gttggttcga ggacctggga 1141 tcaaaccaaa tcagacaagc aagatgctgg ttgccaacat tgacttgggt cctactattt 1201 tggacattgc tggctacgac ctaaataaga cacagatgga tgggatgtcc ttattgccca 1261 ttttgagagg tgccagtaac ttgacctggc gatcagatgt cctggtggaa taccaaggag 1321 aaggccgtaa cgtcactgac ccaacatgcc cttccctgag tcctggcgta TCTCAATGCT 1381 TCCCAGACTG TGTATGTGAA GATGCTTATA ACAATACCTA TGCCTGTGTG AGGACAATGT 1441 CAGCATTGTG GAATTTGCAG TATTGCGAGT TTGATGACCA GGAGGTGTTT GTAGAAGTCT 1501 ATAATCTGAC TGCAGACCCA GACCAGATCA CTAACATTGC TAAAACCATA GACCCAGAGC 1561 TTTTAGGAAA GATGAACTAT CGGTTAATGA TGTTACAGTC CTGTTCTGGG CCAACCTGTC 1621 GCACTCCAGG GGTTTTTGAC CCCGGATACA GGTTTGACCC CCGTCTCATG TTCAGCAATC 1681 GCGGCAGTGT CAGGACTCGA AGATTTTCCA AACATCTTCT GTACCCAACT TTCTTGTACA 1741 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 1801 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 1861 AGGACGAATT TACCAATGCC CGATTCTAAA CACGCGTTAA GTCgacaatc aacctctgga 1921 ttacaaaatt tgtgaaagat t