Construct: ORF TRCN0000477260
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000596.1_s317c1
- Derived from:
- ccsbBroadEn_05755
- DNA Barcode:
- CTTATGCAAAGGACTCTCGTTCGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ABO (28)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477260
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 28 | ABO | ABO, alpha 1-3-N-acetylgala... | NM_020469.3 | 99.2% | 98.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1128
- ORF length:
- 1062
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc cgaggtgttg cggacgctgg ccggaaaacc aaaatgccac gcacttcgac 121 ctatgatcct tttcctaata atgcttgtct tggtcttgtt tggttacggg gtcctaagcc 181 ccagaagtct aatgccagga agcctggaac gggggttctg catggctgtt agggaacctg 241 accatctgca gcgcgtctcg ttgccaagga tggtctaccc ccagccaaag gtgctgacac 301 cgtgtaggaa ggatgtcctc gtggtgaccc cttggctggc tcccattgtc tgggagggca 361 cgttcaacat cgacatcctc aacgagcagt tcaggctcca gaacaccacc attgggttaa 421 ctgtgtttgc catcaagaaa tacgtggctt tcctgaagct gttcctggag acggcggaga 481 agcacttcat ggtgggccac cgtgtccact actatgtctt caccgaccag ccggccgcgg 541 tgccccgcgt gacgctgggg accggtcggc agctgtcagt gctggaggtg ggcgcctaca 601 agcgctggca ggacgtgtcc atgcgccgca tggagatgat cagtgacttc tgcgagcggc 661 gcttcctcag cgaggtggat tacctggtgt gcgtggacgt ggacatggag ttccgcgacc 721 atgtgggcgt ggagaTCCTG ACTCCGCTGT TCGGCACCCT GCACCCCAGC TTCTACGGAA 781 GCAGCCGGGA GGCCTTCACC TACGAGCGCC GGCCCCAGTC CCAGGCCTAC ATCCCCAAGG 841 ACGAGGGCGA TTTCTACTAC ATGGGGGCGT TCTTCGGGGG GTCGGTGCAA GAGGTGCAGC 901 GGCTCACCAG GGCCTGCCAC CAGGCCATGA TGGTCGACCA GGCCAACGGC ATCGAGGCCG 961 TGTGGCACGA CGAGAGCCAC CTGAACAAGT ACCTACTGCG CCACAAACCC ACCAAGGTGC 1021 TCTCCCCCGA GTACTTGTGG GACCAACAGC TGCTGGGCTG GCCCGCCGTC CTGAGGAAGC 1081 TGAGGTTCAC TGCGGTGCCC AAGAACCACC AGGCGGTCCG GAACCCGTGC CCAACTTTCT 1141 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 1201 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGCTTT ATATATCTTG 1261 TGGAAAGGAC GACTTATGCA AAGGACTCTC GTTCGCACGC GTTAAGTCga caatcaacct 1321 ctggattaca aaatttgtga aagatt