Transcript: Human NM_020469.3

Homo sapiens ABO, alpha 1-3-N-acetylgalactosaminyltransferase and alpha 1-3-galactosyltransferase (ABO), mRNA.

Source:
NCBI, updated 2019-09-18
Taxon:
Homo sapiens (human)
Gene:
ABO (28)
Length:
6478
CDS:
13..1077

Additional Resources:

NCBI RefSeq record:
NM_020469.3
NBCI Gene record:
ABO (28)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_020469.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000035244 GCCATCAAGAAATACGTGGCT pLKO.1 376 CDS 100% 0.660 0.924 N ABO n/a
2 TRCN0000431862 AGAACGGGTAAACTCTGTTTC pLKO_005 1143 3UTR 100% 10.800 8.640 N ABO n/a
3 TRCN0000428630 TGCTTGTCTTGGTCTTGTTTG pLKO_005 89 CDS 100% 10.800 7.560 N ABO n/a
4 TRCN0000035246 CACTTCGACCTATGATCCTTT pLKO.1 59 CDS 100% 4.950 3.465 N ABO n/a
5 TRCN0000035245 CCGTGTCCACTACTATGTCTT pLKO.1 447 CDS 100% 4.950 3.465 N ABO n/a
6 TRCN0000035247 CATGGAGATGATCAGTGACTT pLKO.1 576 CDS 100% 4.950 2.970 N ABO n/a
7 TRCN0000035248 CGAGCAGTTCAGGCTCCAGAA pLKO.1 330 CDS 100% 1.350 0.810 N ABO n/a
8 TRCN0000168774 GAGATGGAGTTTCACCATGTT pLKO.1 5389 3UTR 100% 4.950 2.475 Y LOC400464 n/a
9 TRCN0000130146 CAGGTTCAAGTGATTCTCCTA pLKO.1 5296 3UTR 100% 2.640 1.320 Y DICER1-AS1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_020469.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_05755 pDONR223 100% 99.2% 98.8% None (many diffs) n/a
2 ccsbBroad304_05755 pLX_304 0% 99.2% 98.8% V5 (many diffs) n/a
3 TRCN0000477260 CTTATGCAAAGGACTCTCGTTCGC pLX_317 37.2% 99.2% 98.8% V5 (many diffs) n/a
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