Construct: ORF TRCN0000477273
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003579.1_s317c1
- Derived from:
- ccsbBroadEn_06074
- DNA Barcode:
- TACCCCATGCTGAATCCCTATTGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CYP2B6 (1555)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477273
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1555 | CYP2B6 | cytochrome P450 family 2 su... | NM_000767.5 | 99.7% | 99.5% | 516G>T;714G>A;785A>G |
| 2 | human | 1556 | CYP2B7P | cytochrome P450 family 2 su... | NR_001278.1 | 47.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1542
- ORF length:
- 1473
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggaactcagc gtcctcctct tccttgcact cctcacagga ctcttgctac 121 tcctggttca gcgccaccct aacacccatg accgcctccc accagggccc cgccctctgc 181 cccttttggg aaaccttctg cagatggata gaagaggcct actcaaatcc tttctgaggt 241 tccgagagaa atatggggac gtcttcacgg tacacctggg accgaggccc gtggtcatgc 301 tgtgtggagt agaggccata cgggaggccc ttgtggacaa ggctgaggcc ttctctggcc 361 ggggaaaaat cgccatggtc gacccattct tccggggata tggtgtgatc tttgccaatg 421 gaaaccgctg gaaggtgctt cggcgattct ctgtgaccac tatgagggac ttcgggatgg 481 gaaagcggag tgtggaggag cggattcagg aggaggctca gtgtctgata gaggagcttc 541 ggaaatccaa gggggccctc atggacccca ccttcctctt ccattccatt accgccaaca 601 tcatctgctc catcgtcttt ggaaaacgat tccactacca agatcaagag ttcctgaaga 661 tgctgaactt gttctaccag actttttcac tcatcagctc tgtattcggc cagctgtttg 721 agctcttctc tggcttcttg aaatactttc ctggggcaca caggcaagtt tacaaaaacc 781 tacaggaaat caatgcttac attggccaca gtgtggagaa gcaccgtgaa accctggacc 841 ccagcgcccc cagggacctc atcgacacct acctgctcca catggaaaaa gagaaatcca 901 acgcacacag tgaattcagc caccagaacc tcaacctcaa cacgctctcg ctcttctttg 961 ctggcactga gaccaccagc accactctcc gctacggctt cctgctcatg ctcaaatacc 1021 ctcatgttgc agagagagtc tacagggaga ttgaacaggt gattggccca catcgccctc 1081 cagagcttca tgaccgagcc aaaatgccat acacagaggc agtcatctat gagattcaga 1141 gattttccga ccttctcccc atgggtgtgc cccacattgt cacccaacac accagcttcc 1201 gagggtacat catccccaag gacacagaag tatttctcat ccTGAGCACT GCTCTCCATG 1261 ACCCACACTA CTTTGAAAAA CCAGACGCCT TCAATCCTGA CCACTTTCTG GATGCCAATG 1321 GGGCACTGAA AAAGACTGAA GCTTTTATCC CCTTCTCCTT AGGGAAGCGG ATTTGTCTTG 1381 GTGAAGGCAT CGCCCGTGCG GAATTGTTCC TCTTCTTCAC CACCATCCTC CAGAACTTCT 1441 CCATGGCCAG CCCCGTGGCC CCAGAAGACA TCGATCTGAC ACCCCAGGAG TGTGGTGTGG 1501 GCAAAATACC CCCAACATAC CAGATCCGCT TCCTGCCCCG CTTGCCAACT TTCTTGTACA 1561 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 1621 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 1681 AGGACGATAC CCCATGCTGA ATCCCTATTG TACGCGTTAA GTCgacaatc aacctctgga 1741 ttacaaaatt tgtgaaagat t