Construct: ORF TRCN0000477276
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005953.1_s317c1
- Derived from:
- ccsbBroadEn_13430
- DNA Barcode:
- CCGCTTTACCCGCGTGCGTCAAGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TWISTNB (221830)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477276
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 221830 | TWISTNB | TWIST neighbor | NM_001002926.2 | 66.6% | 66.5% | 669_670insA;678_1014del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 747
- ORF length:
- 678
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggctgcaggt tgctcagagg cgccgcggcc agcggcggct tctgatgggt 121 ctctggtagg gcaggctggc gtcctgcctt gcctagagtt gccgacttat gccgctgctt 181 gtgcgctggt gaacagtcgc tactcatgcc tggtggccgg gccgcaccaa aggcacatcg 241 cgctgtcgcc ccgctacctt aacaggaaac gcaccggcat tcgagaacag cttgatgcgg 301 agctccttcg ctattctgag agccttttag gtgtccctat tgcatatgat aacatcaaag 361 ttgtgggaga gcttGGAGAT ATTTATGATG ATCAAGGACA CATTCATCTT AACATTGAAG 421 CCGATTTTGT TATTTTCTGC CCTGAACCAG GGCAGAAGCT TATGGGTATA GTTAATAAAG 481 TGTCTTCTAG CCACATTGGC TGTTTAGTAC ATGGGTGTTT CAATGCCTCC ATTCCTAAAC 541 CTGAGCAGTT GTCAGCTGAG CAGTGGCAAA CCATGGAGAT AAACATGGGT GATGAACTAG 601 AATTTGAAGT ATTTCGTTTA GACTCAGATG CTGCTGGAGT ATTCTGCATT CGGGGAAAAC 661 TAAATATCAC AAGTTTACAA TTCAAGCGCT CTGAAGTTTC TGAAGAAGTT ACAGAAAATG 721 GCACTGAGGA AGCTGCTAAA AAAACCTTGC CAACTTTCTT GTACAAAGTG GTTGATATCG 781 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 841 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GACCGCTTTA 901 CCCGCGTGCG TCAAGAACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 961 aagatt