Construct: ORF TRCN0000477283
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008935.1_s317c1
- Derived from:
- ccsbBroadEn_03823
- DNA Barcode:
- AGCCCGACGAAGATAGAGGTATTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ISY1 (57461)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477283
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57461 | ISY1 | ISY1 splicing factor homolog | NM_020701.4 | 100% | 100% | |
| 2 | human | 57461 | ISY1 | ISY1 splicing factor homolog | NM_001199469.2 | 92.8% | 92.5% | 419_484del |
| 3 | human | 100534599 | ISY1-RAB43 | ISY1-RAB43 readthrough | NM_001204890.1 | 86% | 85.8% | 852C>G;854_857delGCAG;860_993del |
| 4 | mouse | 57905 | Isy1 | ISY1 splicing factor homolog | NM_133934.4 | 89.3% | 98.2% | (many diffs) |
| 5 | mouse | 57905 | Isy1 | ISY1 splicing factor homolog | XM_030255520.1 | 56.4% | 61.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 921
- ORF length:
- 855
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ccgaaatgca gaaaaggcca tgacggcctt agcaagattt cgccaggctc 121 agctggaaga gggaaaagtg aaggaacgaa gaccctttct ggcctcagaa tgtactgaac 181 tgcctaaagc tgagaagtgg agacgacaga tcattggaga gatctctaaa aaagtggctc 241 agattcagaa tgctggttta ggtgaatttc gaattcgtga cctgaatgat gaaattaaca 301 agctgctaag ggagaaagga cactgggagg tccggataaa ggagctggga ggtcctgatt 361 atggaaaagt tggccctaaa atgctggatc atgaaggaaa agaagtccca ggaaaccgag 421 gttacaagta ctttggagca gcaaaagatt tgcctggtgt tagagagctg tttgaaaaag 481 aacctcttcc tcctcccaga aagacacgtg ctgagctcat gaaggcaatc gattttgagt 541 actatggtta ccTAGATGAA GATGATGGTG TTATTGTGCC TTTGGAACAG GAATATGAAA 601 AGAAACTCAG AGCCGAGTTA GTGGAAAAGT GGAAAGCAGA GAGAGAGGCT CGGCTGGCAA 661 GAGGAGAAAA GGAAGAGGAG GAGGAAGAGG AGGAAGAGAT CAACATCTAT GCAGTCACCG 721 AGGAGGAGTC GGACGAGGAA GGCAGCCAGG AGAAAGGAGG GGACGACAGC CAGCAGAAGT 781 TCATTGCTCA CGTCCCTGTT CCCTCGCAGC AAGAGATTGA GGAGGCACTG GTGCGAAGGA 841 AGAAAATGGA ACTCCTCCAG AAGTATGCAA GCGAGACCCT GCAGGCCCAA AGTGAAGAAG 901 CCAGAAGGCT CCTGGGGTAT TACCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 961 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 1021 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAAGCC CGACGAAGAT 1081 AGAGGTATTT ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt