Construct: ORF TRCN0000477288
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009732.1_s317c1
- Derived from:
- ccsbBroadEn_12097
- DNA Barcode:
- CTTAAATCATCAATGGTCTGTATT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PGPEP1 (54858)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477288
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54858 | PGPEP1 | pyroglutamyl-peptidase I | NM_001329477.2 | 100% | 100% | |
| 2 | human | 54858 | PGPEP1 | pyroglutamyl-peptidase I | NM_001329478.2 | 100% | 100% | |
| 3 | human | 54858 | PGPEP1 | pyroglutamyl-peptidase I | NM_017712.4 | 63.1% | 63.1% | 1_231del |
| 4 | human | 54858 | PGPEP1 | pyroglutamyl-peptidase I | NM_001308366.2 | 44.9% | 33.6% | 1_231del;434_435ins40;513_514ins74 |
| 5 | human | 54858 | PGPEP1 | pyroglutamyl-peptidase I | NM_001329471.2 | 44.2% | 28.9% | (many diffs) |
| 6 | human | 54858 | PGPEP1 | pyroglutamyl-peptidase I | NR_138029.2 | 4.9% | 1_436del;639_640ins40;793_7162del | |
| 7 | mouse | 66522 | Pgpep1 | pyroglutamyl-peptidase I | XM_006509719.3 | 62.5% | 71.1% | (many diffs) |
| 8 | mouse | 66522 | Pgpep1 | pyroglutamyl-peptidase I | NM_023217.4 | 50.8% | 57.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 465
- ORF length:
- 396
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttGGCACCAT GGCGACCACA GTCACACTGG AGAAATGTGG ACACAACAAG GGCTACAAGG 121 GGCTGGACAA CTGCCGCTTT TGCCCCGGCT CCCAGTGCTG CGTGGAGGAC GGGCCTGAAA 181 GCATTGACTC CATCATCGAC ATGGATGCTG TGTGCAAGCG AGTCACCACG TTGGGCCTGG 241 ATGTGTCGGT GACCATCTCG CAGGATGCCG GCAGATATCT CTGCGACTTT ACCTACTACA 301 CCTCTTTGTA CCAGAGTCAC GGTCGATCAG CCTTCGTCCA CGTGCCCCCA CTGGGGAAGC 361 CGTACAACGC GGACCAGCTG GGCAGGGCAC TGAGAGCCAT CATTGAGGAG ATGTTGGACC 421 TCCTGGAGCA GTCAGAGGGC AAAATCAACT ATTGCCACAA ACACTTGCCA ACTTTCTTGT 481 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 541 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 601 GAAAGGACGA CTTAAATCAT CAATGGTCTG TATTACGCGT TAAGTCgaca atcaacctct 661 ggattacaaa atttgtgaaa gatt