Construct: ORF TRCN0000477306
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003924.1_s317c1
- Derived from:
- ccsbBroadEn_03333
- DNA Barcode:
- CTGACTAACTTATCCGAGTTTTAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ACKR4 (51554)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477306
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51554 | ACKR4 | atypical chemokine receptor 4 | NM_016557.4 | 100% | 100% | |
| 2 | human | 51554 | ACKR4 | atypical chemokine receptor 4 | NM_178445.2 | 100% | 100% | |
| 3 | mouse | 252837 | Ackr4 | atypical chemokine receptor 4 | NM_145700.2 | 83.3% | 85.4% | (many diffs) |
| 4 | mouse | 252837 | Ackr4 | atypical chemokine receptor 4 | XM_006511734.3 | 83.3% | 85.4% | (many diffs) |
| 5 | mouse | 252837 | Ackr4 | atypical chemokine receptor 4 | XM_006511735.3 | 83.3% | 85.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1116
- ORF length:
- 1050
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc tttggaacag aaccagtcaa cagattatta ttatgaggaa aatgaaatga 121 atggcactta tgactacagt caatatgaac tgatctgtat caaagaagat gtcagagaat 181 ttgcaaaagt tttcctccct gtattcctca caatagtttt cgtcattgga cttgcaggca 241 attccatggt agtggcaatt tatgcctatt acaagaaaca gagaaccaaa acagatgtgt 301 acatcctgaa tttggctgta gcagatttac tccttctatt cactctgcct ttttgggctg 361 ttaatgcagt tcatgggtgg gttttaggga aaataatgtg caaaataact tcagccttgt 421 acacactaaa ctttgtctct ggaatgcagt ttctggcttg tatcagcata gacagatatg 481 tggcagtaac taaagtcccc agccaatcag gagtgggaaa accatgctgg atcatctgtt 541 tctgtgtctg gatggctgcc atcttgctga gcatacccca gctggttttt tatacagtaa 601 atgacaatgc taggtgcatt cccattttcc cccgctacct aggaacatca atgaaagcat 661 tgattcaaat gctagagatc tgcattGGAT TTGTAGTACC CTTTCTTATT ATGGGGGTGT 721 GCTACTTTAT CACAGCAAGG ACACTCATGA AGATGCCAAA CATTAAAATA TCTCGACCCC 781 TAAAAGTTCT GCTCACAGTC GTTATAGTTT TCATTGTCAC TCAACTGCCT TATAACATTG 841 TCAAGTTCTG CCGAGCCATA GACATCATCT ACTCCCTGAT CACCAGCTGC AACATGAGCA 901 AACGCATGGA CATCGCCATC CAAGTCACAG AAAGCATCGC ACTCTTTCAC AGCTGCCTCA 961 ACCCAATCCT TTATGTTTTT ATGGGAGCAT CTTTCAAAAA CTACGTTATG AAAGTGGCCA 1021 AGAAATATGG GTCCTGGAGA AGACAGAGAC AAAGTGTGGA GGAGTTTCCT TTTGATTCTG 1081 AGGGTCCTAC AGAGCCAACC AGTACTTTTA GCATTTACCC AACTTTCTTG TACAAAGTGG 1141 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 1201 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 1261 ACTGACTAAC TTATCCGAGT TTTAGACGCG TTAAGTCgac aatcaacctc tggattacaa 1321 aatttgtgaa agatt