Construct: ORF TRCN0000477309
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013200.1_s317c1
- Derived from:
- ccsbBroadEn_14982
- DNA Barcode:
- ACGAAGACCGATTTCTCGTACGCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CKAP4 (10970)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477309
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10970 | CKAP4 | cytoskeleton associated pro... | NM_006825.4 | 99.7% | 99.6% | (many diffs) |
| 2 | mouse | 216197 | Ckap4 | cytoskeleton-associated pro... | NM_175451.1 | 81.6% | 80.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1875
- ORF length:
- 1806
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gccctcggcc aaacaaaggg gctccaaggg cggccacggc gccgcgagcc 121 cctcggagaa gggtgcccac ccgtcgggcg gcgcggatga cgtggcgaag aagccgccgc 181 cggcgccgca gcagccgccg ccgccgcccg cgccgcaccc gcagcagcac ccgcagcagc 241 acccgcagaa ccaggcgcac ggcaagggcg gccaccgcgg cggcggcggc ggcggcggca 301 agtcctcctc ctcctcctcc gccgccggcg ccgcagcagc cgccgccgcc tcgtcctcgg 361 cgtcctgctc gcgcaggctc ggcagggcgc tcaactttct cttctacctc gccctggtgg 421 cggcggccgc tttctcgggc tggtgcgtcc accacgtcct ggaggaggtc cagcaggtcc 481 ggcgcagcca ccaggacttc tcccggcaga gggaggagct gggccagggc ttgcagggcg 541 tcgagcagaa ggtgcagtct ttgcaagcca catttggaac ttttgagtcc atcttgagaa 601 gctcccaaca taaacaagac ctcacagaga aagctgtgaa gcaaggggag agtgaggtca 661 gccggatcag cgaagtgctg cagaaactcc agaatgagat tctcaaagac ctctcggatg 721 ggatccatgt ggtgaaggac gcccgggagc gggacttcac gtccctggag aacacggtgg 781 aggagcggct gacggagctc accaaatcca tcaacgacaa catcgccatc ttcacagaag 841 tccagaagag gagccagaag gagatcaatg acatgaaggc aaaggttgcc tccctggaag 901 aatctgaggg gaacaagcag gatttgaaag ccttaaagga agctgtgaag gagatacaga 961 cctcagccaa gtccagagag tgggacatgg aggccctgag aagtaccctt cagactatgg 1021 agtctgacat ctacaccgag gtccgcgagc tggtgagcct caagcaggag cagcaggctt 1081 tcaaggaggc ggccgacacg gagcggctcg ccctgcaggc cctcacggag aagcttctca 1141 ggtctgagga gtccgtctcc cgcctcccgg aggagatccg gagactggag gaagagctcc 1201 gccagctgaa gtccgattcc cacgggccga aggaggacgg aggcttcaga cactcggaag 1261 cctttgaggc actccagcaa aagagtcagg gactggactc caggctccag cacgtggagg 1321 atggggtgct ctccatgcag gtggcttctg cgcgccagac cgagagcctg gagtccctcc 1381 tgtccaagag ccaggagcac gagcagcgcc tggccgccct gcaggggcgc ctggaaggcc 1441 tcgggtcctc agaggcagac caggatggcc tggccagcac ggtgaggagc ctgggcgaga 1501 cccagctggt gctcTACGGT GACGTGGAGG AGCTGAAGAG GAGTGTGGGC GAGCTCCCCA 1561 GCACCGTGGA ATCACTCCAG AAGGTGCAGG AGCAGGTGCA CACGCTGCTC AGTCAGGACC 1621 AAGCCCAGGC CGCCCGTCTG CCTCCTCAGG ACTTCCTGGA CAGACTTTCT TCTCTAGACA 1681 ACCTGAAAGC CTCAGTCAGC CAAGTGGAGG CGGACTTGAA AATGCTCAGG ACTGCTGTGG 1741 ACAGTTTGGT TGCATACTCG GTCAAAATAG AAACCAACGA GAACAATCTG GAATCAGCCA 1801 AGGGTTTACT AGATGACCTG AGGAATGATC TGGATAGGTT GTTTGTGAAA GTGGAGAAGA 1861 TTCACGAAAA GGTCTTGCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 1921 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 1981 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA ACGAAGACCG ATTTCTCGTA 2041 CGCGACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt