Construct: ORF TRCN0000477339
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002652.1_s317c1
- Derived from:
- BRDN0000391429
- DNA Barcode:
- GATTCCTCCATCTGCGCGCGGGGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ZNF30 (90075)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477339
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 90075 | ZNF30 | zinc finger protein 30 | XM_017027428.1 | 99.3% | 98.3% | (many diffs) |
| 2 | human | 90075 | ZNF30 | zinc finger protein 30 | NM_194325.3 | 86.2% | 85.2% | (many diffs) |
| 3 | human | 90075 | ZNF30 | zinc finger protein 30 | XM_017027425.1 | 86.2% | 85.2% | (many diffs) |
| 4 | human | 90075 | ZNF30 | zinc finger protein 30 | XM_017027426.1 | 86.2% | 85.2% | (many diffs) |
| 5 | human | 90075 | ZNF30 | zinc finger protein 30 | NM_001099437.2 | 86.1% | 85% | (many diffs) |
| 6 | human | 90075 | ZNF30 | zinc finger protein 30 | NM_001099438.1 | 86.1% | 85% | (many diffs) |
| 7 | human | 90075 | ZNF30 | zinc finger protein 30 | XM_011527443.2 | 86.1% | 85% | (many diffs) |
| 8 | human | 90075 | ZNF30 | zinc finger protein 30 | XM_011527444.1 | 86.1% | 85% | (many diffs) |
| 9 | human | 90075 | ZNF30 | zinc finger protein 30 | XM_017027423.1 | 86.1% | 85% | (many diffs) |
| 10 | human | 90075 | ZNF30 | zinc finger protein 30 | XM_017027424.1 | 86.1% | 85% | (many diffs) |
| 11 | human | 90075 | ZNF30 | zinc finger protein 30 | NR_024018.2 | 60.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1695
- ORF length:
- 1626
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggaaccaaaa gatttgcagt tggaagatga tacaatcggc tgtaaagaaa 121 tgcccacctc tgaaaactgt ccatcttttg ctctacatca gaaaataagt agacagaaac 181 cacgtgaatg tcaggaatat ggaaagaccc ttagtcgaga ctcaaagcct gttcaacatg 241 aaagaataca tagtagtgaa aaacccaaca gatgtaaaga atgtgggaag aactttagta 301 atggacatca actcaccata catcagagat tgcatgttgg tgagaaaccc tataaatatg 361 aaaaatgtgg gaaggccttt atcagtggct cagcctttgt taggcatggg agaattcaca 421 ctggtgagaa gccactcaaa tgtaagcaat gtggaaagac tattagcggt agctatcaac 481 ttacagtaca taagagtatt catactggga agaaaccata tgagtgcggg gaatgttgga 541 aagcttttct agtatatgga aagcttaccc ggcatcagag tactcacact ggtgaaaaac 601 cctttgggtg tgaggagtgt gggaaggcct tcagtacctt ttcatacctg gttcaacatc 661 agcgaattca taccagtgaa aaaccttacg aatgcaaaga atgtgggaag gcctttagca 721 ctagctcacc ccttgttaag catcagagaa ttcatactgg cgagaaaccc tatgaatgta 781 aggagtgtgg gaagtccttc actgtgtatg gacagcttac tcgacatcag agtattcata 841 ctggtgagaa accttttgaa tgtaaggaat gtggaaaggc ctttagactt agttccttcc 901 ttcatgcaca tcagcgaatt catgcagaga taaagcccta cggatgcaag gaatgcggga 961 aaaccttcag tcgtgcctca tatcttgttc aacatggaag acttcacact ggcgagaagc 1021 cctatgaatg taaggagtgt ggcaaggcct ttagtactgg ctcatacctt gttcagcatc 1081 agaggatcca tactggggag aaaccctatg aatgtaagga atgtggcaaa gcctttatta 1141 gtcgccatca gcttaccgta catcaaaggg ttcatactgg agagaaaccc tatgagtgta 1201 aggaatgtgg caaggccttc agagtgcacg tacatctcac acagcatcgg aaaattcata 1261 ctgatgtaaa gccctatgaa tgtcaggaat atggaaagac ccTTAGTCGA GCCTCGTACC 1321 TTGTACAACA TAGCAGAATC CATACTGGTA AGAAGCCCTA TGAGTGTAAG GAGTGTGGCA 1381 AGGCCTTCAG TTCTGGCTCA TACCTTGTTC AGCATCAAAG AATTCATACT GGGGAGAAAC 1441 CCTATGAATG TAACAAATGT GGGAAAGCCT TTACTGTTTA TGGACAACTT ATTGGACATC 1501 AGAGTGTTCA CACTGGTGAG AAACCTTTTG AATGTAAGGA ATGCGGGAAG GCCTTTAGAC 1561 TTAATTCATT CCTTACTGAA CATCAGCGGG TACACACTGG TGAGAAACCC TTTAAATGCA 1621 AAAAATGTGG GAAGACCTTT AGATACAGTT CAGCCCTTAA AGTGCATCTG AGAAAACATA 1681 TGAGTGTTAT ACCCTTGCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 1741 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 1801 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA GATTCCTCCA TCTGCGCGCG 1861 GGGCACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt