Transcript: Human XM_017027425.1

PREDICTED: Homo sapiens zinc finger protein 30 (ZNF30), transcript variant X5, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
ZNF30 (90075)
Length:
2643
CDS:
438..2309

Additional Resources:

NCBI RefSeq record:
XM_017027425.1
NBCI Gene record:
ZNF30 (90075)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_017027425.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000015122 CAAGACTCAAAGCCTGTTCAA pLKO.1 828 CDS 100% 4.950 6.930 N ZNF30 n/a
2 TRCN0000015121 GCCTCGTACCTTGTACAACAT pLKO.1 1923 CDS 100% 4.950 6.930 N ZNF30 n/a
3 TRCN0000230776 GGTCTATTCTCATCTTATAAT pLKO_005 2413 3UTR 100% 15.000 10.500 N ZNF30 n/a
4 TRCN0000230773 ATGGGACATTCCCGTTCTAAA pLKO_005 594 CDS 100% 13.200 9.240 N ZNF30 n/a
5 TRCN0000015120 GATGATACAATCGGCTGTAAA pLKO.1 708 CDS 100% 13.200 9.240 N ZNF30 n/a
6 TRCN0000218508 CAGTTGGAAGATGATACAATC pLKO_005 699 CDS 100% 10.800 7.560 N ZNF30 n/a
7 TRCN0000230775 CTCGTACCTTGTACAACATAG pLKO_005 1925 CDS 100% 10.800 7.560 N ZNF30 n/a
8 TRCN0000015119 GCTATCAACTTACAGTACATA pLKO.1 1084 CDS 100% 5.625 3.938 N ZNF30 n/a
9 TRCN0000015118 GCCTTTACTGTTTATGGACAA pLKO.1 2079 CDS 100% 4.050 2.835 N ZNF30 n/a
10 TRCN0000230774 GATCGCCTTATTGGAACAATG pLKO_005 623 CDS 100% 10.800 6.480 N ZNF30 n/a
11 TRCN0000151775 CCCTATGAATGTAAGGAATGT pLKO.1 1716 CDS 100% 4.950 2.475 Y ZNF829 n/a
12 TRCN0000160334 CCTATGAATGTAAGGAATGTA pLKO.1 1717 CDS 100% 5.625 2.813 Y ZNF570 n/a
13 TRCN0000152739 GTAAGGAATGTGGAAAGGCTT pLKO.1 1474 CDS 100% 2.640 1.320 Y ZNF829 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017027425.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_09286 pDONR223 100% 99.7% 99.6% None 159_160insGCA;1474A>G n/a
2 ccsbBroad304_09286 pLX_304 0% 99.7% 99.6% V5 159_160insGCA;1474A>G n/a
3 TRCN0000477793 CCGACTCCTTGTTAGTGGTATGTC pLX_317 20.6% 99.7% 99.6% V5 159_160insGCA;1474A>G n/a
4 TRCN0000477339 GATTCCTCCATCTGCGCGCGGGGC pLX_317 24.4% 86.2% 85.2% V5 (many diffs) n/a
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