Construct: ORF TRCN0000477354
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010192.1_s317c1
- Derived from:
- ccsbBroadEn_09441
- DNA Barcode:
- GTCCAAGCCGGCATAATTGCACTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- THEM4 (117145)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477354
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 117145 | THEM4 | thioesterase superfamily me... | NM_053055.5 | 99.7% | 99.5% | 50T>G;210C>T |
| 2 | human | 117145 | THEM4 | thioesterase superfamily me... | XM_011509142.2 | 73.1% | 73.3% | 0_1ins192;18C>T |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 789
- ORF length:
- 720
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gctgaggagc tgcgccgcgc gcctccgcac gctgggggct ctgtgccggc 121 cgccagtagg ccggcgcctg ccgggaagcg agccgcgacc cgagctgagg tcattttctt 181 ctgaggaagt cattcttaag gactgttctg tccccaaccc cagctggaac aaggacctaa 241 gactgctctt tgaccagttt atgaagaaat gtgaagatgg ctcctggaaa cgtttgcctt 301 catataaacg tacacctact gaatGGATTC AAGACTTCAA AACCCATTTT CTTGACCCAA 361 AGCTTATGAA AGAAGAACAA ATGTCACAGG CCCAGCTCTT CACCAGAAGC TTTGATGATG 421 GCCTGGGCTT TGAATACGTG ATGTTCTACA ATGACATTGA GAAAAGGATG GTTTGCTTAT 481 TTCAAGGAGG CCCTTACCTG GAAGGACCAC CTGGATTCAT TCATGGAGGT GCCATTGCAA 541 CCATGATTGA TGCTACTGTT GGTATGTGTG CAATGATGGC TGGGGGAATC GTCATGACTG 601 CCAATCTCAA CATCAATTAT AAAAGACCTA TCCCTCTTTG TTCTGTTGTT ATGATAAATA 661 GCCAACTTGA TAAAGTTGAA GGAAGGAAAT TTTTTGTTTC CTGTAATGTT CAGAGTGTTG 721 ATGAGAAGAC CCTATACTCA GAGGCGACAA GCTTATTTAT AAAGCTGAAT CCTGCTAAAA 781 GTCTGACATT GCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 841 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 901 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAGTCCAA GCCGGCATAA TTGCACTCAC 961 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt