Construct: ORF TRCN0000477384
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001446.1_s317c1
- Derived from:
- ccsbBroadEn_13220
- DNA Barcode:
- GCGCTAGGGCGTACATTGCGTGTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SLC2A14 (144195)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477384
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 144195 | SLC2A14 | solute carrier family 2 mem... | NM_001286234.2 | 100% | 100% | |
| 2 | human | 144195 | SLC2A14 | solute carrier family 2 mem... | NM_001286235.2 | 100% | 100% | |
| 3 | human | 144195 | SLC2A14 | solute carrier family 2 mem... | XM_005253315.4 | 100% | 100% | |
| 4 | human | 144195 | SLC2A14 | solute carrier family 2 mem... | XM_005253317.5 | 100% | 100% | |
| 5 | human | 144195 | SLC2A14 | solute carrier family 2 mem... | XM_011520562.1 | 100% | 100% | |
| 6 | human | 144195 | SLC2A14 | solute carrier family 2 mem... | XM_011520563.2 | 100% | 100% | |
| 7 | human | 144195 | SLC2A14 | solute carrier family 2 mem... | XM_017018846.1 | 100% | 100% | |
| 8 | human | 144195 | SLC2A14 | solute carrier family 2 mem... | XM_017018847.1 | 100% | 100% | |
| 9 | human | 144195 | SLC2A14 | solute carrier family 2 mem... | XM_024448849.1 | 100% | 100% | |
| 10 | human | 6515 | SLC2A3 | solute carrier family 2 mem... | NM_006931.3 | 96.5% | 94.3% | (many diffs) |
| 11 | human | 144195 | SLC2A14 | solute carrier family 2 mem... | XM_017018841.1 | 95.3% | 95.3% | 19_90del |
| 12 | human | 144195 | SLC2A14 | solute carrier family 2 mem... | XM_017018844.1 | 95.3% | 95.3% | 19_90del |
| 13 | human | 144195 | SLC2A14 | solute carrier family 2 mem... | XM_017018845.2 | 95.3% | 95.3% | 19_90del |
| 14 | human | 144195 | SLC2A14 | solute carrier family 2 mem... | XM_024448848.1 | 95.3% | 95.3% | 19_90del |
| 15 | human | 144195 | SLC2A14 | solute carrier family 2 mem... | NM_001286233.2 | 95.3% | 94.6% | (many diffs) |
| 16 | human | 144195 | SLC2A14 | solute carrier family 2 mem... | NM_153449.4 | 95.3% | 94.6% | (many diffs) |
| 17 | human | 144195 | SLC2A14 | solute carrier family 2 mem... | NM_001286237.2 | 92.6% | 92.1% | (many diffs) |
| 18 | human | 144195 | SLC2A14 | solute carrier family 2 mem... | NM_001286236.2 | 82.5% | 81.8% | (many diffs) |
| 19 | human | 144195 | SLC2A14 | solute carrier family 2 mem... | XM_011520564.2 | 82.5% | 81.8% | (many diffs) |
| 20 | human | 144195 | SLC2A14 | solute carrier family 2 mem... | XM_011520565.2 | 82.5% | 81.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1560
- ORF length:
- 1491
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggacaacaga cagaatgtca ccccagctct gatctttgcc atcacagttg 121 ctacaatcgg ctctttccag tttggctaca acactggggt catcaatgct cctgagacga 181 tcataaagga atttatcaat aaaactttga cggacaaggc aaatgcccct ccctctgagg 241 tgctgctcac gaatctctgg tccttgtctg tggccatatt ttccgtcggg ggtatgatcg 301 gctccttttc cgtcggactc tttgttaacc gctttggcag gcgcaattca atgctgattg 361 tcaacctgtt ggctgccact ggtggctgcc ttatgggact gtgtaaaata gctgagtcag 421 ttgaaatgct gatcctgggc cgcttggtta ttggcctctt ctgcggactc tgcacaggtt 481 ttgtgcccat gtacattgga gagatctcgc ctactgccct gaggggtgcc tttggcactc 541 tcaaccagct gggcatagtt attggaattc tggtggccca gatctttggt ctggaactca 601 tccttgggtc tgaagagcta tggccggtgc tattaggctt taccatcctt ccagctatcc 661 tgcaaagtgc agcccttcca tgttgccctg aaagtcccag atttttgctc attaacagaa 721 aaaaagagga gaatgctacg cggatcctcc agcggttgtg gggcacccag gatgtatccc 781 aagacatcca ggagatgaaa gatgagagtg caaggatgtc acaagaaaag caagtcaccg 841 tgctggagct ctttagagtg tccagctacc gacagcccat catcatttcc attgtgctcc 901 agctctctca gcagctctct gggatcaatg ctgtgttcta ttactcaaca ggaatcttca 961 aggatgcagg tgttcaacag cccatctatg ccaccatcag cgcgggtgtg gttaatacta 1021 tcttcacttt actttctcta tttctggtgg aaagggcagg aagaaggact ctgcatatga 1081 taggccttgg agggatggct ttttgttcca cgctcatgac tgtttctttg ttattaaaga 1141 atcactataa tgggatgagc tttgtctgta ttGGGGCTAT CTTGGTCTTT GTGGCCTGTT 1201 TTGAAATTGG ACCAGGCCCC ATTCCCTGGT TTATTGTGGC CGAACTCTTC AGCCAGGGCC 1261 CCCGCCCAGC TGCGATGGCA GTGGCCGGCT GCTCCAACTG GACCTCCAAC TTCCTAGTCG 1321 GATTGCTCTT CCCCTCTGCT GCTTACTATT TAGGAGCCTA CGTTTTTATT ATCTTCACCG 1381 GCTTCCTCAT TACCTTCTTG GCCTTTACCT TCTTCAAAGT CCCTGAGACC CGTGGCAGGA 1441 CTTTTGAGGA TATCACACGG GCCTTTGAAG GGCAGGCACA CGGTGCAGAT AGATCTGGGA 1501 AGGACGGCGT CATGGGGATG AACAGCATCG AGCCTGCTAA GGAGACCACC ACCAATGTCT 1561 TGCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 1621 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 1681 TTTATATATC TTGTGGAAAG GACGAGCGCT AGGGCGTACA TTGCGTGTAA CGCGTTAAGT 1741 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt