Construct: ORF TRCN0000477417
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015577.1_s317c1
- Derived from:
- ccsbBroadEn_06168
- DNA Barcode:
- CTGCCGATGCAGGTGTCCGCCCGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EPHA3 (2042)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477417
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2042 | EPHA3 | EPH receptor A3 | NM_005233.6 | 99.9% | 99.7% | 1822G>A;2059G>A |
| 2 | human | 2042 | EPHA3 | EPH receptor A3 | XM_005264715.3 | 99.8% | 99.6% | 1429_1430insAGC;1819G>A;2056G>A |
| 3 | human | 2042 | EPHA3 | EPH receptor A3 | XM_005264716.3 | 93.2% | 92.9% | (many diffs) |
| 4 | human | 2042 | EPHA3 | EPH receptor A3 | NM_182644.3 | 54.6% | 53.9% | (many diffs) |
| 5 | mouse | 13837 | Epha3 | Eph receptor A3 | NM_010140.3 | 88.7% | 96.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 3015
- ORF length:
- 2949
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga ttgtcagctc tccatcctcc tccttctcag ctgctctgtt ctcgacagct 121 tcggggaact gattccgcag ccttccaatg aagtcaatct actggattca aaaacaattc 181 aaggggagct gggctggatc tcttatccat cacatgggtg ggaagagatc agtggtgtgg 241 atgaacatta cacacccatc aggacttacc aggtgtgcaa tgtcatggac cacagtcaaa 301 acaattggct gagaacaaac tgggtcccca ggaactcagc tcagaagatt tatgtggagc 361 tcaagttcac tctacgagac tgcaatagca ttccattggt tttaggaact tgcaaggaga 421 cattcaacct gtactacatg gagtctgatg atgatcatgg ggtgaaattt cgagagcatc 481 agtttacaaa gattgacacc attgcagctg atgaaagttt cactcaaatg gatcttgggg 541 accgtattct gaagctcaac actgagatta gagaagtagg tcctgtcaac aagaagggat 601 tttatttggc atttcaagat gttggtgctt gtgttgcctt ggtgtctgtg agagtatact 661 tcaaaaagtg cccatttaca gtgaagaatc tggctatgtt tccagacacg gtacccatgg 721 actcccagtc cctggtggag gttagagggt cttgtgtcaa caattctaag gaggaagatc 781 ctccaaggat gtactgcagt acagaaggcg aatggcttgt acccattggc aagtgttcct 841 gcaatgctgg ctatgaagaa agaggtttta tgtgccaagc ttgtcgacca ggtttctaca 901 aggcattgga tggtaatatg aagtgtgcta agtgcccgcc tcacagttct actcaggaag 961 atggttcaat gaactgcagg tgtgagaata attacttccg ggcagacaaa gaccctccat 1021 ccatggcttg tacccgacct ccatcttcac caagaaatgt tatctctaat ataaacgaga 1081 cctcagttat cctggactgg agttggcccc tggacacagg aggccggaaa gatgttacct 1141 tcaacatcat atgtaaaaaa tgtgggtgga atataaaaca gtgtgagcca tgcagcccaa 1201 atgtccgctt cctccctcga cagtttggac tcaccaacac cacggtgaca gtgacagacc 1261 ttctggcaca tactaactac acctttgaga ttgatgccgt taatggggtg tcagagctga 1321 gctccccacc aagacagttt gctgcggtca gcatcacaac taatcaggct gctccatcac 1381 ctgtcctgac gattaagaaa gatcggacct ccagaaatag catctctttg tcctggcaag 1441 aacctgaaca tcctaatggg atcatattgg actacgaggt caaatactat gaaaagcagg 1501 aacaagaaac aagttatacc attctgaggg caagaggcac aaatgttacc atcagtagcc 1561 tcaagcctga cactatatac gtattccaaa tccgagcccg aacagccgct ggatatggga 1621 cgaacagccg caagtttgag tttgaaacta gtccagactc tttctccatc tctggtgaaa 1681 gtagccaagt ggtcatgatc gccatttcag cggcagtagc aattattctc ctcactgttg 1741 tcatctatgt tttgattggg aggttctgtg gctataagtc aaaacatggg gcagatgaaa 1801 aaagacttca ttttggcaat gggcatttaa aacttccagg tctcaggact tatgttgacc 1861 cacatacata tgaagaccct acccaaactg ttcatgagtt tgccaaggaa ttggatgcca 1921 ccaacatatc cattgataaa gttgttggag caggtgaatt tggagaggtg tgcagtggtc 1981 gcttaaaact tccttcaaaa aaagagattt cagtggccat taagaccctg aaagttggct 2041 acacagaaaa gcagaggaga gacttcctgg gagaagcaag cattatggga cagtttgacc 2101 accccaatat cattcgactg gaaagagttg ttaccaaaag taagccagtt atgattgtca 2161 cagaatacat ggagaatggt tccttggata gtttcctacg taaacacgat gcccagttta 2221 ctgtcattca gctagtgggg atgcttcgag ggatagcatc tggcatgaag tacctgtcag 2281 acatgggcta tgttcaccga gacctcgctg ctcggaacat cttgatcaac agtaacttgg 2341 tgtgtaaggt ttctgatttc ggactttcgc gtgtcctgga ggatgaccca gaagctgctt 2401 atacaacaag aggagggaag atcccaatca ggtggacatc accagaagct atagcctacc 2461 gcaagttcac gtcagccagc gatgtatgga gttatgggat tgttctctgg gaggtgatgt 2521 cttatggaga gagaccatac tgggagatgt ccaaTCAGGA TGTAATTAAA GCTGTAGATG 2581 AGGGCTATCG ACTGCCACCC CCCATGGACT GCCCAGCTGC CTTGTATCAG CTGATGCTGG 2641 ACTGCTGGCA GAAAGACAGG AACAACAGAC CCAAGTTTGA GCAGATTGTT AGTATTCTGG 2701 ACAAGCTTAT CCGGAATCCC GGCAGCCTGA AGATCATCAC CAGTGCAGCC GCAAGGCCAT 2761 CAAACCTTCT TCTGGACCAA AGCAATGTGG ATATCACTAC CTTCCGCACA ACAGGTGACT 2821 GGCTTAATGG TGTCTGGACA GCACACTGCA AGGAAATCTT CACGGGTGTG GAGTACAGTT 2881 CTTGTGACAC AATAGCCAAG ATTTCCACAG ATGACATGAA AAAGGTTGGT GTCACCGTGG 2941 TTGGGCCACA GAAGAAGATC ATCAGTAGCA TTAAAGCTCT AGAAACGCAA TCAAAGAATG 3001 GCCCAGTTCC CGTGTACCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 3061 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 3121 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA CTGCCGATGC AGGTGTCCGC 3181 CCGGACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt