Construct: ORF TRCN0000477430
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006125.1_s317c1
- Derived from:
- ccsbBroadEn_06404
- DNA Barcode:
- TTGCTCCGTCCTTAGTTATGGAAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HSD17B1 (3292)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477430
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3292 | HSD17B1 | hydroxysteroid 17-beta dehy... | NM_000413.4 | 99.8% | 99.6% | 937G>A |
| 2 | human | 3292 | HSD17B1 | hydroxysteroid 17-beta dehy... | NM_001330219.3 | 99.5% | 99.3% | 540_542delCAG;940G>A |
| 3 | human | 3292 | HSD17B1 | hydroxysteroid 17-beta dehy... | NR_144397.2 | 69.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1053
- ORF length:
- 984
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcccgcacc gtggtgctca tcaccggctg ttcctcgggc atcggcctgc 121 acttggccgt acgtctggct tcagatccat cccagagctt caaagtgtat gccacgttga 181 gggacctgaa aacacagggc cggctgtggg aggcggcccg ggccctggca tgccctccgg 241 gatccctgga gacgttgcag ctggacgtaa gggactcaaa atccgtggcc gctgcccggg 301 aacgcgtgac tgagggccgc gtggacgtgc tggtgtgtaa cgcaggcctg ggcctgctgg 361 ggccgctgga ggcgctgggg gaggacgccg tggcctctgt gctggacgtg aatgtagtag 421 ggactgtgcg gatgctgcag gccttcctgc cagacatgaa gaggcgcggt tcgggacgcg 481 tgttggtgac cgggagcgtg ggaggattga tggggctgcc tttcaatgac gtttattgcg 541 ccagcaagtt cgcgctcgaa ggcttatgcg agagtctggc ggttctgctg ctgccctttg 601 gggtccactt gagcctgatc gagtgcggcc cagtgcacac cgccttcatg gagaaggtgt 661 tgggcagccc agaggaggtg ctggaccgca cggacatcca caccttccac cgcttctacc 721 aatacctcgc ccacagcaag caagtctttc gcgaggcggc gcagaacccT GAGGAGGTGG 781 CGGAGGTCTT CCTCACCGCT TTGCGCGCCC CGAAGCCGAC CCTGCGCTAC TTCACCACCG 841 AGCGCTTCCT GCCCCTGCTG CGGATGCGCC TGGACGACCC CAGCGGCTCC AACTACGTCA 901 CCGCCATGCA CCGGGAAGTG TTCGGCGACG TTCCGGCAAA GGCCGAGGCT GGGGCCGAGG 961 CTGGGGGCGG GGCCGGGCCT GGGGCAGAGG ACGAGGCCGG GCGCAGTGCG GTGGGGGACC 1021 CTGAGCTCGG CGATCCTCCG GCCGCCCCGC AGTTGCCAAC TTTCTTGTAC AAAGTGGTTG 1081 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 1141 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGATT 1201 GCTCCGTCCT TAGTTATGGA AGACGCGTTA AGTCgacaat caacctctgg attacaaaat 1261 ttgtgaaaga tt