Construct: ORF TRCN0000477455
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003387.1_s317c1
- Derived from:
- ccsbBroadEn_02780
- DNA Barcode:
- AACATGTCTGCTTTTGGTTACTTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CES3 (23491)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477455
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23491 | CES3 | carboxylesterase 3 | NM_001185177.2 | 100% | 100% | |
| 2 | human | 23491 | CES3 | carboxylesterase 3 | NM_024922.6 | 99.4% | 99.4% | 1441_1449delGCCTTTCCA |
| 3 | human | 23491 | CES3 | carboxylesterase 3 | NM_001185176.1 | 36.2% | 36.2% | 0_1ins1083;358_366delGCCTTTCCA |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1773
- ORF length:
- 1704
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 tgggcaccat ggagagagca gtgagagtgg agtccggggt cctggtcggg gtggtctgtc 121 tgctcctggc atgccctgcc acagccactg ggcccgaagt tgctcagcct gaagtagaca 181 ccaccctggg tcgtgtgcga ggccggcagg tgggcgtgaa gggcacagac cgccttgtga 241 atgtctttct gggcattcca tttgcccagc cgccactggg ccctgaccgg ttctcagccc 301 cacacccagc acagccctgg gagggtgtgc gggatgccag cactgcgccc ccaatgtgcc 361 tacaagacgt ggagagcatg aacagcagca gatttgtcct caacggaaaa cagcagatct 421 tctccgtttc agaggactgc ctggtcctca acgtctatag cccagctgag gtccccgcag 481 ggtccggtag gccggtcatg gtatgggtcc atggaggcgc tctgataact ggcgctgcca 541 cctcctacga tggatcagct ctggctgcct atggggatgt ggtcgtggtt acagtccagt 601 accgccttgg ggtccttggc ttcttcagca ctggagatga gcatgcacct ggcaaccagg 661 gcttcctaga tgtggtagct gctttgcgct gggtgcaaga aaacatcgcc cccttcgggg 721 gtgacctcaa ctgtgtcact gtctttggtg gatctgccgg tgggagcatc atctctggcc 781 tggtcctgtc cccagtggct gcagggctgt tccacagagc catcacacag agtggggtca 841 tcaccacccc agggatcatc gactctcacc cttggcccct agctcagaaa atcgcaaaca 901 ccttggcctg cagctccagc tccccggctg agatggtgca gtgccttcag cagaaagaag 961 gagaagagct ggtccttagc aagaagctga aaaatactat ctatcctctc accgttgatg 1021 gcactgtctt ccccaaaagc cccaaggaac tcctgaagga gaagcccttc cactctgtgc 1081 ccttcctcat gggtgtcaac aaccatgagt tcagctggct catccccagg ggctggggtc 1141 tcctggatac aatggagcag atgagccggg aggacatgct ggccatctca acacccgtct 1201 tgaccagtct ggatgtgccc cctgagatga tgcccaccgt catagatgaa tacctaggaa 1261 gcaactcgga cgcacaagcc aaatgccagg cgttccagga attcatgggt gacgtattca 1321 tcaatgttcc caccgtcagt ttttcaagat accTTCGAGA TTCTGGAAGC CCTGTCTTTT 1381 TCTATGAGTT CCAGCATCGA CCCAGTTCTT TTGCGAAGAT CAAACCTGCC TGGGTGAAGG 1441 CTGATCATGG GGCCGAGGGT GCTTTTGTGT TCGGAGGTCC CTTCCTCATG GACGAGAGCT 1501 CCCGCCTGGA GGCCACAGAG GAGGAGAAGC AGCTAAGCCT CACCATGATG GCCCAGTGGA 1561 CCCACTTTGC CCGGACAGGG GACCCCAATA GCAAGGCTCT GCCTCCTTGG CCCCAATTCA 1621 ACCAGGCGGA ACAATATCTG GAGATCAACC CAGTGCCACG GGCCGGACAG AAGTTCAGGG 1681 AGGCCTGGAT GCAGTTCTGG TCAGAGACGC TCCCCAGCAA GATACAACAG TGGCACCAGA 1741 AGCAGAAGAA CAGGAAGGCC CAGGAGGACC TCTTGCCAAC TTTCTTGTAC AAAGTGGTTG 1801 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 1861 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAAA 1921 CATGTCTGCT TTTGGTTACT TAACGCGTTA AGTCgacaat caacctctgg attacaaaat 1981 ttgtgaaaga tt