Construct: ORF TRCN0000477475
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005978.1_s317c1
- Derived from:
- ccsbBroadEn_14060
- DNA Barcode:
- CTACCCTCTCCAGGGCCCTCTATC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MID2 (11043)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477475
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 11043 | MID2 | midline 2 | XM_017029239.2 | 99.9% | 99.8% | 2048C>T |
| 2 | human | 11043 | MID2 | midline 2 | NM_052817.3 | 97.1% | 97% | 1_60del;2108C>T |
| 3 | human | 11043 | MID2 | midline 2 | XM_005262062.5 | 95.7% | 95.6% | 1286_1375del;2138C>T |
| 4 | human | 11043 | MID2 | midline 2 | NM_012216.4 | 93.1% | 93% | 1_60del;1346_1435del;2198C>T |
| 5 | mouse | 23947 | Mid2 | midline 2 | NM_011845.2 | 94% | 99.2% | (many diffs) |
| 6 | mouse | 23947 | Mid2 | midline 2 | XM_017318482.1 | 94% | 99.2% | (many diffs) |
| 7 | mouse | 23947 | Mid2 | midline 2 | XM_006528523.3 | 91.3% | 96.4% | (many diffs) |
| 8 | mouse | 23947 | Mid2 | midline 2 | XM_006528524.3 | 90% | 95.1% | (many diffs) |
| 9 | mouse | 23947 | Mid2 | midline 2 | XM_006528522.3 | 87.6% | 92.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2121
- ORF length:
- 2055
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga aacactggag tctgaattga cctgtccaat ctgcctagag ttgtttgaag 121 acccccttct gctcccttgt gctcacagcc tctgcttcag ctgtgcccat cgcattttgg 181 tatcaagctg cagctctggt gaatccattg aacccattac tgctttccag tgtcctacct 241 gcaggtatgt tatctcgctg aaccaccggg gcctggatgg cctcaagagg aatgtgactc 301 tgcagaacat tattgatcgc ttccagaagg cttcagtcag tgggcccaat tcccctagtg 361 agagccgccg ggaaaggact tacaggccca ccactgccat gtctagcgag cgaattgctt 421 gccaattctg tgagcaggac ccgccaaggg atgcagtaaa aacatgcatc acctgtgagg 481 tctcctactg tgaccgttgc ctgcgggcca cgcaccccaa caagaaacct ttcaccagcc 541 accgcctggt ggaaccagtg ccagacacac atcttcgagg gatcacctgc ctggaccatg 601 agaatgagaa agtgaacatg tactgtgtat ctgatgacca attgatctgt gccttatgca 661 aactggtggg tcgtcaccga gaccatcagg tcgcatccct gaatgatcga tttgagaaac 721 tcaagcaaac tctggagatg aacctcacca acctggttaa gcgcaacagc gaactagaaa 781 atcaaatggc caaactaata cagatctgcc agcaggttga ggtgaatact gctatgcatg 841 aggcaaaact tatggaagaa tgtgacgagt tggtagagat catccagcag aggaagcaaa 901 tgatcgctgt caaaatcaaa gagacaaagg ttatgaaact gagaaagttg gcacagcagg 961 ttgctaattg ccgccagtgt cttgaacggt caacagtcct catcaaccaa gctgagcata 1021 tcctgaaaga aaatgaccag gcacggtttc tacagtctgc aaaaaatatt gctgagaggg 1081 tcgctatggc aactgcatct tctcaagttc tgattccaga catcaatttt aatgatgcct 1141 ttgaaaactt tgctttagat ttttccagag aaaagaaact gctagagggg ttagattatt 1201 taacagcccc aaacccacca tctatccgag aagaactctg tactgcctcc catgacacca 1261 ttacagtcca ctggatctcg gatgatgagt tcagcatcag ctcctatgag cttcagtaca 1321 ccatattcac tggccaggct aacttcatca gcctgtataa ttcagtagac agctggatga 1381 ttgttcccaa cattaaacag aaccattaca cagtgcatgg actccagagc gggactcgct 1441 acatcttcat cgttaaagcc ataaaccaag ccggcagccg gaacagtgaa cctacccgac 1501 taaaaacaaa cagccaaccc tttaaattgg atcccaaaat gactcacaag aagttgaaga 1561 tctccaatga tggattgcag atggagaagg atgaaagctc tctaaagaag agccacaccc 1621 cagagaggtt tagtggcaca gggtgctatg gggcagcagg aaatatattc attgacagtg 1681 gctgccacta ttgggaggtg gtcatgggtt cctcaacatg gtatgcaatt ggcattgcct 1741 acaaatcagc tccaaagaat gaatggattG GCAAGAATGC CTCCTCATGG GTCTTCTCTC 1801 GCTGCAATAG TAACTTCGTG GTGAGACACA ACAACAAGGA AATGCTGGTG GATGTGCCCC 1861 CACACCTGAA GCGTCTGGGT GTCCTCCTGG ATTATGACAA CAATATGCTG TCTTTCTATG 1921 ACCCAGCTAA CTCTCTCCAT CTTCATACTT TTGATGTGAC CTTCATTCTT CCAGTTTGTC 1981 CAACATTTAC AATCTGGAAC AAATCCCTAA TGATCCTGTC TGGCTTGCCT GCCCCAGATT 2041 TTATTGATTA CCCTGAGCGG CAGGAATGCA ACTGCAGGCC TCAAGAATCC CCTTATGTTT 2101 CTGGGATGAA AATCTGTCAT TACCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 2161 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 2221 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGACTAC CCTCTCCAGG 2281 GCCCTCTATC ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt