Transcript: Human NM_052817.3

Homo sapiens midline 2 (MID2), transcript variant 2, mRNA.

Source:
NCBI, updated 2019-08-09
Taxon:
Homo sapiens (human)
Gene:
MID2 (11043)
Length:
7243
CDS:
562..2679

Additional Resources:

NCBI RefSeq record:
NM_052817.3
NBCI Gene record:
MID2 (11043)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_052817.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000425003 CACCTAAGTTAGCGTTCAATA pLKO_005 2737 3UTR 100% 13.200 18.480 N MID2 n/a
2 TRCN0000430061 GTGAATCCATTGAACCCATTA pLKO_005 755 CDS 100% 10.800 15.120 N MID2 n/a
3 TRCN0000033768 GCAGAACATTATTGATCGCTT pLKO.1 858 CDS 100% 2.640 3.696 N MID2 n/a
4 TRCN0000033766 CGAGCGAATTGCTTGCCAATT pLKO.1 963 CDS 100% 1.080 1.512 N MID2 n/a
5 TRCN0000033765 CCACCATCTATCCGAGAAGAA pLKO.1 1771 CDS 100% 4.950 3.960 N MID2 n/a
6 TRCN0000415228 TAAGTTTCAGGAGAGTATATA pLKO_005 2677 CDS 100% 15.000 10.500 N MID2 n/a
7 TRCN0000428613 CAATCTGCCTAGAGTTGTTTG pLKO_005 653 CDS 100% 10.800 7.560 N MID2 n/a
8 TRCN0000033764 CCCTTATGTTTCTGGGATGAA pLKO.1 2646 CDS 100% 4.950 3.465 N MID2 n/a
9 TRCN0000033767 CCTCCTGGATTATGACAACAA pLKO.1 2439 CDS 100% 4.950 3.465 N MID2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_052817.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_14060 pDONR223 100% 97.1% 97% None 1_60del;2108C>T n/a
2 ccsbBroad304_14060 pLX_304 0% 97.1% 97% V5 1_60del;2108C>T n/a
3 TRCN0000477475 CTACCCTCTCCAGGGCCCTCTATC pLX_317 17.4% 97.1% 97% V5 1_60del;2108C>T n/a
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