Construct: ORF TRCN0000477489

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF016078.1_s317c1
Derived from:
ccsbBroadEn_00273
DNA Barcode:
ATTTTAAAACAAGAACTGGCAATT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
CDC25B (994)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000477489
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 994 CDC25B cell division cycle 25B NM_021873.3 100% 100%
2 human 994 CDC25B cell division cycle 25B NM_004358.4 97.5% 97.5% 196_197ins42
3 human 994 CDC25B cell division cycle 25B NM_021872.3 92.9% 92.9% 459_460ins123
4 human 994 CDC25B cell division cycle 25B NM_001287516.1 88.9% 88.9% 0_1ins174;5_6insCGACCTCGCCGGGCTCGG
5 human 994 CDC25B cell division cycle 25B NM_001287517.1 86.3% 86.2% 1_1delAins235;3G>C;5A>G
6 human 994 CDC25B cell division cycle 25B NM_001287518.1 84.3% 84.3% 0_1ins174;5_6insCGACCTCGCCGGGCTCGG;644_645ins81
7 human 994 CDC25B cell division cycle 25B NM_001287519.1 80.6% 80.6% 0_1ins336
8 human 994 CDC25B cell division cycle 25B NM_001287520.1 80.6% 80.6% 0_1ins336
9 human 994 CDC25B cell division cycle 25B NM_001287522.1 73.6% 73.6% 0_1ins336;123_124ins123
10 human 994 CDC25B cell division cycle 25B NM_001287524.1 66.5% 66.5% 0_1ins580;3_6delGCCG
11 human 994 CDC25B cell division cycle 25B XR_937181.3 57% 1_69del;1262_1340del;1889_3048del
12 human 994 CDC25B cell division cycle 25B NR_136335.1 54.2% (many diffs)
13 human 994 CDC25B cell division cycle 25B NR_136336.1 52.9% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1806
ORF length:
1740
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgga ggtgccccag ccggagcccg cgccaggctc ggctctcagt ccagcaggcg
121tgtgcggtgg cgcccagcgt ccgggccacc tcccgggcct cctgctggga tctcatggcc
181tcctggggtc cccggtgcgg gcggccgctt cctcgccggt caccaccctc acccagacca
241tgcacgacct cgccgggctc ggcagcgaaa ccccaaagag tcaggtaggg accctgctct
301tccgcagccg cagccgcctg acgcacctat ccctgtctcg acgggcatcc gaatcctccc
361tgtcgtctga atcctccgaa tcttctgatg caggtctctg catggattcc cccagcccta
421tggaccccca catggcggag cagacgtttg aacaggccat ccaggcagcc agccggatca
481ttcgaaacga gcagtttgcc atcagacgct tccagtctat gccggtgagg ctgctgggcc
541acagccccgt gcttcggaac atcaccaact cccaggcgcc cgacggccgg aggaagagcg
601aggcgggcag tggagctgcc agcagctctg gggaagacaa ggagaatgat ggatttgtct
661tcaagatgcc atggaagccc acacatccca gctccaccca tgctctggca gagtgggcca
721gccgcaggga agcctttgcc cagagaccca gctcggcccc cgacctgatg tgtctcagtc
781ctgaccggaa gatggaagtg gaggagctca gccccctggc cctaggtcgc ttctctctga
841cccctgcaga gggggatact gaggaagatg atggatttgt ggacatccta gagagtgact
901taaaggatga tgatgcagtt cccccaggca tggagagtct cattagtgcc ccactggtca
961agaccttgga aaaggaagag gaaaaggacc tcgtcatgta cagcaagtgc cagcggctct
1021tccgctctcc gtccatgccc tgcagcgtga tccggcccat cctcaagagg ctggagcggc
1081cccaggacag ggacacgccc gtgcagaata agcggaggcg gagcgtgacc cctcctgagg
1141agcagcagga ggctgaggaa cctaaagccc gcgtcctccg ctcaaaatca ctgtgtcacg
1201atgagatcga gaacctcctg gacagtgacc accgagagct gattggagat tactctaagg
1261ccttcctcct acagacagta gacggaaagc accaagacct caagtacatc tcaccagaaa
1321cgatggtggc cctattgacg ggcaagttca gcaacatcgt ggataagttt gtgattgtag
1381actgcagata cccctatgaa tatgaaggcg ggcacatcaa gactgcggtg aacttgcccc
1441tggaacgcga cgccgagagc ttccTACTGA AGAGCCCCAT CGCGCCCTGT AGCCTGGACA
1501AGAGAGTCAT CCTCATTTTC CACTGTGAAT TCTCATCTGA GCGTGGGCCC CGCATGTGCC
1561GTTTCATCAG GGAACGAGAC CGTGCTGTCA ACGACTACCC CAGCCTCTAC TACCCTGAGA
1621TGTATATCCT GAAAGGCGGC TACAAGGAGT TCTTCCCTCA GCACCCGAAC TTCTGTGAAC
1681CCCAGGACTA CCGGCCCATG AACCACGAGG CCTTCAAGGA TGAGCTAAAG ACCTTCCGCC
1741TCAAGACTCG CAGCTGGGCT GGGGAGCGGA GCCGGCGGGA GCTCTGTAGC CGGCTGCAGG
1801ACCAGTGCCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC
1861TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT
1921CTTGGCTTTA TATATCTTGT GGAAAGGACG AATTTTAAAA CAAGAACTGG CAATTACGCG
1981TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt

Download FASTA (ORF) (Full)