Construct: ORF TRCN0000477513
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009706.1_s317c1
- Derived from:
- ccsbBroadEn_10228
- DNA Barcode:
- TGCCCGTGGCAAGAAAGTAGCAAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CMAHP (8418)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477513
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8418 | CMAHP | cytidine monophospho-N-acet... | NR_027626.1 | 41.2% | 1_531del;1273_1795del | |
| 2 | human | 8418 | CMAHP | cytidine monophospho-N-acet... | NR_002174.2 | 29% | (many diffs) | |
| 3 | mouse | 12763 | Cmah | cytidine monophospho-N-acet... | NM_001111110.2 | 37.4% | 35.6% | (many diffs) |
| 4 | mouse | 12763 | Cmah | cytidine monophospho-N-acet... | NM_001284519.1 | 37.4% | 35.6% | (many diffs) |
| 5 | mouse | 12763 | Cmah | cytidine monophospho-N-acet... | NM_007717.5 | 37.4% | 35.6% | (many diffs) |
| 6 | mouse | 12763 | Cmah | cytidine monophospho-N-acet... | XM_011244265.2 | 37.2% | 35.4% | (many diffs) |
| 7 | mouse | 12763 | Cmah | cytidine monophospho-N-acet... | XM_011244266.2 | 37.2% | 35.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 810
- ORF length:
- 741
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggatgaaaac aacggacttt tgcttttaga actgaatcct cctaaccctt 121 gggacttaca gcccagatct cctgaagagt tggcttttgg agaagtacag ataacatatc 181 tcactcatgc ctgcatggac ctcaagttag gagacaagag aatggtgttt gacccttggt 241 taatcggtcc tgcttttgcc cgtggatggt ggttgctcca tgagcctcca tctgattggc 301 tggagaggct gtgccaggca gacctcattt acatcagtca tctgcactca gaccacctga 361 gttaccccac actgaaaaag cttgctggga gaagaccaga tattcccatt tatgttggaa 421 acacagaaag gcctgtattt tGGAATCTGA ATCAGAGCGG TGTCCAGTTG ACTAATATCA 481 ATGTCGTGCC ATTTGGAATA TGGCAGCAGG TGGACAAAAA TCTTCGATTC ATGATCTTGA 541 TGGACGGTGT TCATCCTGAG ATGGACACTT GCATTATTGT GGAGTACAAA GGTCATAAAA 601 TACTCAATAT AGTAGACTGC ACCAGACCCA ATGGGGGAAG GCTGCCTATG AAGGTTGCTC 661 TAATGATGAG TGATTTTGCT GGAGGAGCAT CAGGCTTTCC AATGACTTTC AGTGGTGGAA 721 AATTTACGGG TAATATTTTG TATGAAAGTG ATACCAAGTA TCACGCATGG CACATTGCTA 781 AATGCTGTAA GAGTAAATAC AAATGTAATT TGCCAACTTT CTTGTACAAA GTGGTTGATA 841 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 901 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGATGCCC 961 GTGGCAAGAA AGTAGCAAAA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 1021 tgaaagatt