Construct: ORF TRCN0000477521

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF002292.1_s317c1
Derived from:
ccsbBroadEn_11828
DNA Barcode:
GTATTAGGCAAGGCAAAGTCGATC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
MYOF (26509)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000477521
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 26509 MYOF myoferlin XM_017016070.2 79.3% 68.1% (many diffs)
2 human 26509 MYOF myoferlin NM_013451.4 76.5% 76.5% 3670_5121del;5760A>G
3 human 26509 MYOF myoferlin NM_133337.3 75.8% 75.8% 1415_1416ins39;3631_5082del;5721A>G
4 human 26509 MYOF myoferlin XM_005269693.4 75.8% 75.8% 3670_5178del;5817A>G
5 human 26509 MYOF myoferlin XM_005269694.5 75.1% 75.1% 1415_1416ins39;3631_5139del;5778A>G
6 human 26509 MYOF myoferlin XM_011539632.2 74.7% 74.2% (many diffs)
7 human 26509 MYOF myoferlin XM_017016068.2 68.9% 68.9% 0_1ins426;3244_4752del;5391A>G
8 human 26509 MYOF myoferlin XM_017016069.1 68.9% 68.9% 0_1ins426;3244_4752del;5391A>G
9 mouse 226101 Myof myoferlin NM_001302140.1 67.8% 71.7% (many diffs)
10 mouse 226101 Myof myoferlin NM_001099634.1 67.2% 71.1% (many diffs)
11 mouse 226101 Myof myoferlin XM_006526994.1 67.2% 71% (many diffs)
12 mouse 226101 Myof myoferlin XM_006526995.1 66.6% 70.4% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
4797
ORF length:
4731
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgct gcgagtgatt gtggaatctg ccagcaatat ccctaaaacg aaatttggca
121agccggatcc tattgtttct gtcattttta aggatgagaa aaagaaaaca aagaaagttg
181ataatgaatt gaaccctgtc tggaatgaga ttttggagtt tgacttgagg ggtataccac
241tggacttttc atcttccctt gggattattg tgaaagattt tgagacaatt ggacaaaata
301aattaattgg cacggcgact gtagccctga aggacctgac tggtgaccag agcagatccc
361tgccgtacaa gctgatctcc ctgctaaatg aaaaagggca agatactggg gccaccattg
421acttggtgat cggctatgat ccgccttctg ctccacatcc aaatgacctg agcgggccca
481gcgtgccagg catgggagga gatggggaag aagatgaagg tgatgaagac aggttggaca
541atgcagtcag gggccctggg cccaaggggc cagttgggac ggtgtcggaa gctcagcttg
601ctcggaggct caccaaagta aagaacagcc ggcggatgct gtcaaataag ccacaggact
661tccagatccg cgtccgagtg attgagggcc gacagttaag tggtaacaac ataaggcctg
721tggtcaaagt tcacgtctgt ggccagacac accgaacaag aatcaagaga ggaaacaacc
781ctttttttga tgagttgttt ttctacaatg tcaacatgac cccttctgaa ttgatggatg
841agatcatcag catccgggtt tataattctc actctctgcg ggcagattgt ctgatggggg
901aatttaagat tgatgttgga tttgtttatg atgaacctgg ccatgctgtc atgagaaagt
961ggcttcttct caatgacccg gaagatacca gttcaggttc taaaggttat atgaaagtca
1021gcatgtttgt cctgggaacc ggagatgagc ctcctcctga gagacgagat cgtgataatg
1081acagtgatga tgtggagagt aatttgttac tccctgctgg cattgccctc cggtgggtga
1141ccttcttgct gaaaatctac cgagctgagg acatccccca gatggatgat gccttctcac
1201agacagtaaa ggaaatattt ggaggcaatg cagataagaa aaatctcgtg gatccttttg
1261tagaagtttc ctttgctgga aaaaaggttt gtacaaacat aattgagaaa aatgcaaacc
1321cagagtggaa tcaggtcgtc aatcttcaga tcaagtttcc ttcagtgtgt gaaaaaataa
1381aactaacaat atatgactgg gaccgtctta ctaaaaatga tgtagttgga acaacatatc
1441tacacctctc taaaattgct gcctctggtg gggaagtgga agatttctca tcttcgggaa
1501ctggggctgc atcatataca gtaaacacag gagaaacaga ggtaggcttt gttccaacgt
1561ttggaccttg ttacctgaat ctttatggaa gccccagaga gtacacggga ttcccagacc
1621cctatgatga gctgaatact ggaaaggggg aaggagttgc ctacagaggc aggatcttgg
1681ttgaattagc cacttttctt gagaagacac caccagataa aaagcttgag cccatttcaa
1741atgatgacct gctggttgtt gagaaatacc agcgaaggcg gaagtacagc ctgtctgccg
1801tgtttcattc agccaccatg ttgcaagatg ttggtgaggc cattcagttt gaagtcagca
1861ttgggaacta tggcaacaag tttgacacca cctgtaagcc tttggcatca acaactcagt
1921acagccgtgc tgtatttgat ggcaactact attattactt gccttgggcc cacaccaagc
1981cagttgttac cctgacttca tactgggagg atattagtca tcgcctggat gcggtgaaca
2041ctctcctagc tatggcagaa cggctgcaaa caaatataga agctctaaaa tcagggatac
2101aaggtaaaat tcctgcaaac cagctggctg aattgtggct gaagctgata gatgaagtta
2161tagaagacac gagatacacg ttgcctctca cagaaggaaa agccaacgtc acagttctcg
2221atactcagat ccgaaagctg cggtccaggt ctctctccca aatacatgag gcggctgtga
2281ggatgaggtc ggaagccaca gatgtgaagt ccacactggc agaaattgag gactggcttg
2341ataaattaat gcagctgact gaagagccac agaacagcat gcctgacatc atcatctgga
2401tgatccgggg agagaagaga ctggcctatg cacgaattcc cgcacatcag gtcttgtact
2461ccaccagtgg tgagaatgca tctggaaaat actgtgggaa aacccaaacc atctttctga
2521agtatccaca ggagaaaaac aacgggccaa aggtgcctgt ggagttgcga gtgaacatct
2581ggctaggctt aagtgctgtg gagaagaagt ttaacagctt cgcagaagga actttcaccg
2641tctttgctga aatgtatgaa aatcaagctc tcatgtttgg aaaatggggt acttctggat
2701tagtaggacg tcataagttt tctgatgtca caggaaaaat aaaactcaag agggaatttt
2761ttctgcctcc aaaaggctgg gaatgggaag gagagtggat agttgatcct gaaagaagct
2821tgctgactga ggcagatgca ggtcacacgg agttcactga tgaagtctat cagaacgaga
2881gccgctaccc cgggggcgac tggaagccgg ccgaggacac ctacacggat gcgaacggcg
2941ataaagcagc atcacccagc gagttgactt gtcctccagg ttgggaatgg gaagatgatg
3001catggtctta tgacataaat cgagcggtgg atgagaaagg ctgggaatat ggaatcacca
3061ttcctcctga tcataagccc aaatcctggg ttgcagcaga gaaaatgtac cacactcata
3121gacggcgaag gctggtccga aaacgcaaga aagatttaac acagactgct tcaagcaccg
3181caagggccat ggaggaattg caagaccaag agggctggga atatgcttct ctaattggct
3241ggaaatttca ctggaaacaa cgtagttcag ataccttccg ccgcagacgc tggaggagaa
3301aaatggctcc ttcagaaaca catggtgcag ctgccatctt taaacttgaa ggtgcccttg
3361gggcagacac taccgaagat ggggatgaga agagcctgga gaaacagaag cacagtgcca
3421ccactgtgtt cggagcaaac acccccattg tttcctgcaa ttttgacaga gtctacatct
3481accatctgcg ctgctatgtc tatcaagcca gaaacctctt ggctttagat aaggatagct
3541tttcagatcc atatgctcat atctgtttcc tccatcggag caaaaccact gagatcatcc
3601attcaaccct gaatcccacg tgggaccaaa caattatatt cgatgaagtt gaaatctatg
3661gggaacccca aacagttcta cagaatccac ccaaagttat catggaactt tttgacaatg
3721accaagtggg caaagatgaa tttgaagcca acaaaatcct gcaccagcac ctcggggccc
3781ctgaagagcg gcttgctctt cacatcctca ggactcaggg gctggtccct gagcacgtgg
3841aaacaaggac tttgcacagc accttccagc ccaacatttc ccagggaaaa cttcagatgt
3901gggtggatgt tttccccaag agtttggggc caccaggccc tcctttcaac atcacacccc
3961ggaaagccaa gaaatactac ctgcgtgtga tcatctggaa caccaaggac gttatcttgg
4021acgagaaaag catcacagga gaggaaatga gtgacatcta cgtcaaaggc tggattcctg
4081gcaatgaaga aaacaaacag aaaacagatg tccattacag atctttggat ggtgaaggga
4141attttaactg gcgatttgtt ttcccgtttg actaccttcc agccgaacaa ctctgtatcg
4201ttgcgaaaaa agagcatttc tggagtattg accaaacgga atttcgaatc ccacccaggc
4261tgatcattca gatatgggac aatgacaagt tttctctgga tgactacttg ggtttcctag
4321aacttgactt gcgtcacacg atcattcctg caaaatcacc agagaaatgc aggttggaca
4381tgattccgga cctcaaagcc atgaaccccc TTAAAGCCAA GACAGCCTCC CTCTTTGAGC
4441AGAAGTCCAT GAAAGGATGG TGGCCATGCT ACGCAGAGAA AGATGGCGCC CGCGTAATGG
4501CTGGGAAAGT GGAGATGACA TTGGAAATCC TCAACGAGAA GGAGGCCGAC GAGAGGCCAG
4561CCGGGAAGGG GCGGGACGAA CCCAACATGA ACCCCAAGCT GGACTTACCA AATCGACCAG
4621AAACCTCCTT CCTCTGGTTC ACCAACCCAT GCAAGACCAT GAAGTTCATC GTGTGGCGCC
4681GCTTTAAGTG GGTCATCATC GGCTTGCTGT TCCTGCTTAT CCTGCTGCTC TTCGTGGCCG
4741TGCTCCTCTA CTCTTTGCCG AACTATTTGT CAATGAAGAT TGTAAAGCCA AATGTGTACC
4801CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC
4861TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT
4921ATATATCTTG TGGAAAGGAC GAGTATTAGG CAAGGCAAAG TCGATCACGC GTTAAGTCga
4981caatcaacct ctggattaca aaatttgtga aagatt

Download FASTA (ORF) (Full)