Construct: ORF TRCN0000477521
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002292.1_s317c1
- Derived from:
- ccsbBroadEn_11828
- DNA Barcode:
- GTATTAGGCAAGGCAAAGTCGATC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MYOF (26509)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477521
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 26509 | MYOF | myoferlin | XM_017016070.2 | 79.3% | 68.1% | (many diffs) |
| 2 | human | 26509 | MYOF | myoferlin | NM_013451.4 | 76.5% | 76.5% | 3670_5121del;5760A>G |
| 3 | human | 26509 | MYOF | myoferlin | NM_133337.3 | 75.8% | 75.8% | 1415_1416ins39;3631_5082del;5721A>G |
| 4 | human | 26509 | MYOF | myoferlin | XM_005269693.4 | 75.8% | 75.8% | 3670_5178del;5817A>G |
| 5 | human | 26509 | MYOF | myoferlin | XM_005269694.5 | 75.1% | 75.1% | 1415_1416ins39;3631_5139del;5778A>G |
| 6 | human | 26509 | MYOF | myoferlin | XM_011539632.2 | 74.7% | 74.2% | (many diffs) |
| 7 | human | 26509 | MYOF | myoferlin | XM_017016068.2 | 68.9% | 68.9% | 0_1ins426;3244_4752del;5391A>G |
| 8 | human | 26509 | MYOF | myoferlin | XM_017016069.1 | 68.9% | 68.9% | 0_1ins426;3244_4752del;5391A>G |
| 9 | mouse | 226101 | Myof | myoferlin | NM_001302140.1 | 67.8% | 71.7% | (many diffs) |
| 10 | mouse | 226101 | Myof | myoferlin | NM_001099634.1 | 67.2% | 71.1% | (many diffs) |
| 11 | mouse | 226101 | Myof | myoferlin | XM_006526994.1 | 67.2% | 71% | (many diffs) |
| 12 | mouse | 226101 | Myof | myoferlin | XM_006526995.1 | 66.6% | 70.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 4797
- ORF length:
- 4731
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgct gcgagtgatt gtggaatctg ccagcaatat ccctaaaacg aaatttggca 121 agccggatcc tattgtttct gtcattttta aggatgagaa aaagaaaaca aagaaagttg 181 ataatgaatt gaaccctgtc tggaatgaga ttttggagtt tgacttgagg ggtataccac 241 tggacttttc atcttccctt gggattattg tgaaagattt tgagacaatt ggacaaaata 301 aattaattgg cacggcgact gtagccctga aggacctgac tggtgaccag agcagatccc 361 tgccgtacaa gctgatctcc ctgctaaatg aaaaagggca agatactggg gccaccattg 421 acttggtgat cggctatgat ccgccttctg ctccacatcc aaatgacctg agcgggccca 481 gcgtgccagg catgggagga gatggggaag aagatgaagg tgatgaagac aggttggaca 541 atgcagtcag gggccctggg cccaaggggc cagttgggac ggtgtcggaa gctcagcttg 601 ctcggaggct caccaaagta aagaacagcc ggcggatgct gtcaaataag ccacaggact 661 tccagatccg cgtccgagtg attgagggcc gacagttaag tggtaacaac ataaggcctg 721 tggtcaaagt tcacgtctgt ggccagacac accgaacaag aatcaagaga ggaaacaacc 781 ctttttttga tgagttgttt ttctacaatg tcaacatgac cccttctgaa ttgatggatg 841 agatcatcag catccgggtt tataattctc actctctgcg ggcagattgt ctgatggggg 901 aatttaagat tgatgttgga tttgtttatg atgaacctgg ccatgctgtc atgagaaagt 961 ggcttcttct caatgacccg gaagatacca gttcaggttc taaaggttat atgaaagtca 1021 gcatgtttgt cctgggaacc ggagatgagc ctcctcctga gagacgagat cgtgataatg 1081 acagtgatga tgtggagagt aatttgttac tccctgctgg cattgccctc cggtgggtga 1141 ccttcttgct gaaaatctac cgagctgagg acatccccca gatggatgat gccttctcac 1201 agacagtaaa ggaaatattt ggaggcaatg cagataagaa aaatctcgtg gatccttttg 1261 tagaagtttc ctttgctgga aaaaaggttt gtacaaacat aattgagaaa aatgcaaacc 1321 cagagtggaa tcaggtcgtc aatcttcaga tcaagtttcc ttcagtgtgt gaaaaaataa 1381 aactaacaat atatgactgg gaccgtctta ctaaaaatga tgtagttgga acaacatatc 1441 tacacctctc taaaattgct gcctctggtg gggaagtgga agatttctca tcttcgggaa 1501 ctggggctgc atcatataca gtaaacacag gagaaacaga ggtaggcttt gttccaacgt 1561 ttggaccttg ttacctgaat ctttatggaa gccccagaga gtacacggga ttcccagacc 1621 cctatgatga gctgaatact ggaaaggggg aaggagttgc ctacagaggc aggatcttgg 1681 ttgaattagc cacttttctt gagaagacac caccagataa aaagcttgag cccatttcaa 1741 atgatgacct gctggttgtt gagaaatacc agcgaaggcg gaagtacagc ctgtctgccg 1801 tgtttcattc agccaccatg ttgcaagatg ttggtgaggc cattcagttt gaagtcagca 1861 ttgggaacta tggcaacaag tttgacacca cctgtaagcc tttggcatca acaactcagt 1921 acagccgtgc tgtatttgat ggcaactact attattactt gccttgggcc cacaccaagc 1981 cagttgttac cctgacttca tactgggagg atattagtca tcgcctggat gcggtgaaca 2041 ctctcctagc tatggcagaa cggctgcaaa caaatataga agctctaaaa tcagggatac 2101 aaggtaaaat tcctgcaaac cagctggctg aattgtggct gaagctgata gatgaagtta 2161 tagaagacac gagatacacg ttgcctctca cagaaggaaa agccaacgtc acagttctcg 2221 atactcagat ccgaaagctg cggtccaggt ctctctccca aatacatgag gcggctgtga 2281 ggatgaggtc ggaagccaca gatgtgaagt ccacactggc agaaattgag gactggcttg 2341 ataaattaat gcagctgact gaagagccac agaacagcat gcctgacatc atcatctgga 2401 tgatccgggg agagaagaga ctggcctatg cacgaattcc cgcacatcag gtcttgtact 2461 ccaccagtgg tgagaatgca tctggaaaat actgtgggaa aacccaaacc atctttctga 2521 agtatccaca ggagaaaaac aacgggccaa aggtgcctgt ggagttgcga gtgaacatct 2581 ggctaggctt aagtgctgtg gagaagaagt ttaacagctt cgcagaagga actttcaccg 2641 tctttgctga aatgtatgaa aatcaagctc tcatgtttgg aaaatggggt acttctggat 2701 tagtaggacg tcataagttt tctgatgtca caggaaaaat aaaactcaag agggaatttt 2761 ttctgcctcc aaaaggctgg gaatgggaag gagagtggat agttgatcct gaaagaagct 2821 tgctgactga ggcagatgca ggtcacacgg agttcactga tgaagtctat cagaacgaga 2881 gccgctaccc cgggggcgac tggaagccgg ccgaggacac ctacacggat gcgaacggcg 2941 ataaagcagc atcacccagc gagttgactt gtcctccagg ttgggaatgg gaagatgatg 3001 catggtctta tgacataaat cgagcggtgg atgagaaagg ctgggaatat ggaatcacca 3061 ttcctcctga tcataagccc aaatcctggg ttgcagcaga gaaaatgtac cacactcata 3121 gacggcgaag gctggtccga aaacgcaaga aagatttaac acagactgct tcaagcaccg 3181 caagggccat ggaggaattg caagaccaag agggctggga atatgcttct ctaattggct 3241 ggaaatttca ctggaaacaa cgtagttcag ataccttccg ccgcagacgc tggaggagaa 3301 aaatggctcc ttcagaaaca catggtgcag ctgccatctt taaacttgaa ggtgcccttg 3361 gggcagacac taccgaagat ggggatgaga agagcctgga gaaacagaag cacagtgcca 3421 ccactgtgtt cggagcaaac acccccattg tttcctgcaa ttttgacaga gtctacatct 3481 accatctgcg ctgctatgtc tatcaagcca gaaacctctt ggctttagat aaggatagct 3541 tttcagatcc atatgctcat atctgtttcc tccatcggag caaaaccact gagatcatcc 3601 attcaaccct gaatcccacg tgggaccaaa caattatatt cgatgaagtt gaaatctatg 3661 gggaacccca aacagttcta cagaatccac ccaaagttat catggaactt tttgacaatg 3721 accaagtggg caaagatgaa tttgaagcca acaaaatcct gcaccagcac ctcggggccc 3781 ctgaagagcg gcttgctctt cacatcctca ggactcaggg gctggtccct gagcacgtgg 3841 aaacaaggac tttgcacagc accttccagc ccaacatttc ccagggaaaa cttcagatgt 3901 gggtggatgt tttccccaag agtttggggc caccaggccc tcctttcaac atcacacccc 3961 ggaaagccaa gaaatactac ctgcgtgtga tcatctggaa caccaaggac gttatcttgg 4021 acgagaaaag catcacagga gaggaaatga gtgacatcta cgtcaaaggc tggattcctg 4081 gcaatgaaga aaacaaacag aaaacagatg tccattacag atctttggat ggtgaaggga 4141 attttaactg gcgatttgtt ttcccgtttg actaccttcc agccgaacaa ctctgtatcg 4201 ttgcgaaaaa agagcatttc tggagtattg accaaacgga atttcgaatc ccacccaggc 4261 tgatcattca gatatgggac aatgacaagt tttctctgga tgactacttg ggtttcctag 4321 aacttgactt gcgtcacacg atcattcctg caaaatcacc agagaaatgc aggttggaca 4381 tgattccgga cctcaaagcc atgaaccccc TTAAAGCCAA GACAGCCTCC CTCTTTGAGC 4441 AGAAGTCCAT GAAAGGATGG TGGCCATGCT ACGCAGAGAA AGATGGCGCC CGCGTAATGG 4501 CTGGGAAAGT GGAGATGACA TTGGAAATCC TCAACGAGAA GGAGGCCGAC GAGAGGCCAG 4561 CCGGGAAGGG GCGGGACGAA CCCAACATGA ACCCCAAGCT GGACTTACCA AATCGACCAG 4621 AAACCTCCTT CCTCTGGTTC ACCAACCCAT GCAAGACCAT GAAGTTCATC GTGTGGCGCC 4681 GCTTTAAGTG GGTCATCATC GGCTTGCTGT TCCTGCTTAT CCTGCTGCTC TTCGTGGCCG 4741 TGCTCCTCTA CTCTTTGCCG AACTATTTGT CAATGAAGAT TGTAAAGCCA AATGTGTACC 4801 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 4861 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 4921 ATATATCTTG TGGAAAGGAC GAGTATTAGG CAAGGCAAAG TCGATCACGC GTTAAGTCga 4981 caatcaacct ctggattaca aaatttgtga aagatt