Transcript: Mouse NM_001302140.1

Mus musculus myoferlin (Myof), transcript variant 1, mRNA.

Source:
NCBI, updated 2017-06-04
Taxon:
Mus musculus (mouse)
Gene:
Myof (226101)
Length:
7133
CDS:
255..6440

Additional Resources:

NCBI RefSeq record:
NM_001302140.1
NBCI Gene record:
Myof (226101)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_001302140.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000027714 CCGTCAGTTATGCGGCAACAA pLKO.1 878 CDS 100% 4.950 6.930 N Myof n/a
2 TRCN0000027699 GATCCTATCGTTTCTGTCATT pLKO.1 315 CDS 100% 4.950 6.930 N Myof n/a
3 TRCN0000027717 CACGAGAATCAAGAGAGGGAA pLKO.1 944 CDS 100% 2.640 3.696 N Myof n/a
4 TRCN0000027726 CCCATTCTTTGAGGGCAGATT pLKO.1 1057 CDS 100% 4.950 3.465 N Myof n/a
5 TRCN0000027739 GAACAGGTCTTTGCCCTACAA pLKO.1 539 CDS 100% 4.950 3.465 N Myof n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001302140.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_11828 pDONR223 100% 67.8% 71.7% None (many diffs) n/a
2 ccsbBroad304_11828 pLX_304 0% 67.8% 71.7% V5 (many diffs) n/a
3 TRCN0000477521 GTATTAGGCAAGGCAAAGTCGATC pLX_317 8.3% 67.8% 71.7% V5 (many diffs) n/a
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