Construct: ORF TRCN0000477555
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004381.1_s317c1
- Derived from:
- ccsbBroadEn_11398
- DNA Barcode:
- AGGCACCGAATTCAGCAATATTTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SAFB2 (9667)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477555
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9667 | SAFB2 | scaffold attachment factor B2 | XM_011528449.3 | 58.4% | 58.4% | 1581G>A;1583A>T;1585_2706del |
| 2 | human | 9667 | SAFB2 | scaffold attachment factor B2 | NM_014649.3 | 55.3% | 55.2% | 1581G>A;1583A>T;1585_2859del |
| 3 | human | 9667 | SAFB2 | scaffold attachment factor B2 | XM_024451801.1 | 15.4% | 15.4% | (many diffs) |
| 4 | human | 9667 | SAFB2 | scaffold attachment factor B2 | XM_024451802.1 | 13.8% | 13.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1650
- ORF length:
- 1584
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggagactctg cccgggtcgg gcgactcggg ccctggcacg gcttctctcg 121 gcccgggcgt tgcggagact gggacgaggc ggctcagcga gctgcgggtg atcgatctgc 181 gggcggagct gaagaagcgg aacctggaca cgggcggcaa caagagcgtc ctgatggagc 241 ggctcaagaa ggcggttaaa gaagaggggc aagatcctga tgaaattggc atcgagttag 301 aagccaccag caagaagtca gccaagagat gtgttaaagg actgaagatg gaggaggaag 361 gcacagaaga taatggcctg gaagacgatt ccagagacgg gcaggaggac atggaagcaa 421 gtctggagaa cctgcagaat atgggcatga tggacatgag tgtgctagac gaaactgaag 481 tggcgaatag cagtgctcca gattttgggg aggatggcac ggacggcctt ctcgattcct 541 tttgtgatag taaagaatac gtggctgcac agctgagaca gctcccggct cagcccccag 601 agcatgctgt ggatggggaa ggatttaaga acactttgga aacttcatcg ttgaacttca 661 aagtaactcc ggacattgaa gaatcccttt tggagccaga aaatgagaaa atactcgaca 721 ttttggggga aacttgtaaa tctgagccag taaaagaaga aagttccgag ctggagcagc 781 catttgcaca ggacacaagt agcgtggggc cagacagaaa gcttgcggag gaagaggacc 841 tatttgacag cgcccatccg gaagagggtg atttagattt ggccagcgag tcaacagcac 901 acgctcagtc gagcaaggca gacagcctgt tagcggtagt gaaaagggag cccgcggagc 961 agccaggcga tggcgagagg acggactgtg agcctgtagg gctagagccg gcagttgagc 1021 agagtagtgc ggcctccgag ctcgcggagg cctctagcga ggagctcgca gaagcaccca 1081 cggaagcccc aagcccagaa gccagagata gcaaagaaga cgggaggaag tttgattttg 1141 acgcttgtaa tgaagtccct ccggctccta aagagtcctc aaccagtgag ggcgctgatc 1201 agaaaatgag ctcttttaag gaagaaaaag atataaagcc aaTCATTAAA GATGAAAAAG 1261 GTCGGGTCGG CAGCGGTTCT GGTCGGAACC TGTGGGTCAG CGGGCTGTCC TCCACAACAC 1321 GCGCTACGGA TCTCAAGAAC CTTTTCAGCA AGTATGGGAA GGTTGTCGGG GCCAAAGTGG 1381 TAACGAACGC CCGCAGCCCG GGGGCTCGAT GCTATGGATT CGTCACCATG TCGACATCTG 1441 ACGAGGCGAC CAAGTGCATC AGCCATCTCC ACAGAACTGA GCTGCATGGA CGAATGATCT 1501 CCGTAGAGAA GGCCAAAAAT GAGCCTGCTG GGAAAAAGCT TTCCGACAGA AAAGAGTGCG 1561 AAGTGAAGAA GGAAAAATTA TCGAGTGTCG ACAGACATCA TTCTGTGGAG ATCAAAATTG 1621 AAAAAACTGT AATTAAGAAG GAAGAAATGT ACCCAACTTT CTTGTACAAA GTGGTTGATA 1681 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 1741 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAAGGCA 1801 CCGAATTCAG CAATATTTTA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 1861 tgaaagatt