Construct: ORF TRCN0000477565
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012775.1_s317c1
- Derived from:
- ccsbBroadEn_05340
- DNA Barcode:
- ATATCGAGCCGCCTATGGCATTTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- OR51B5 (282763)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477565
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 282763 | OR51B5 | olfactory receptor family 5... | NM_001005567.3 | 99.8% | 99.6% | 13G>A |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1005
- ORF length:
- 936
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gtcgtccagc agcagctccc atcccttcct attgactggt tttccaggct 121 tggaggaagc tcatcactgg atttccgtat ttttcttgtt catgtatata tccatccttt 181 ttggcaatgg caccctcctt cttctcatta aggaagatca caatcttcat gagcccatgt 241 acttctttct ggccatgctg gctgccacag acctggggct ggccctgacc acaatgccca 301 cggtgctggg agtcctctgg ctggatcaca gggagattgg aagtgcggcc tgcttttccc 361 aggcctactt tatacactca ctttcctttc tcgagtctgg cattctgctt gccatggcct 421 atgaccgttt tattgccatc tgcaaccctc ttagatatac ctctgtactt actaatactc 481 gagtagtgaa gattgggctg ggagttctga tgaggggatt tgtatccgtt gttcccccaa 541 tcaggcccct ctattttttt ctgtattgtc actcccatgt tctttcacat gcattctgcc 601 ttcaccagga tGTCATTAAA CTCGCCTGTG CTGATACCAC CTTCAACCGA CTGTACCCAG 661 CTGTGCTTGT AGTCTTTATA TTTGTGCTGG ATTATCTGAT TATCTTCATC TCCTATGTGT 721 TGATACTCAA GACTGTCCTG AGCATTGCCT CCAGAGAGGA GAGGGCCAAG GCTCTCATTA 781 CCTGTGTCTC CCATATCTGC TGTGTCCTGG TTTTTTATGT CACAGTGATT GGATTGTCTC 841 TGATTCATCG TTTTGGAAAG CAGGTTCCAC ATATTGTTCA CCTCATTATG AGCTATGCCT 901 ATTTTCTGTT CCCTCCACTA ATGAATCCTA TAACATATAG TGTCAAGACC AAGCAGATTC 961 AGAATGCCAT TCTTCACCTT TTTACTACCC ATAGAATTGG AACCTTGCCA ACTTTCTTGT 1021 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 1081 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 1141 GAAAGGACGA ATATCGAGCC GCCTATGGCA TTTAACGCGT TAAGTCgaca atcaacctct 1201 ggattacaaa atttgtgaaa gatt