Construct: ORF TRCN0000477613
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016597.1_s317c1
- Derived from:
- ccsbBroadEn_05060
- DNA Barcode:
- GTGGCCATGGTCTTCGTCTAACTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PPP4R2 (151987)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477613
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 151987 | PPP4R2 | protein phosphatase 4 regul... | NM_174907.4 | 100% | 100% | |
| 2 | human | 151987 | PPP4R2 | protein phosphatase 4 regul... | NM_001318026.1 | 90.8% | 90.8% | 0_1ins114 |
| 3 | human | 151987 | PPP4R2 | protein phosphatase 4 regul... | NM_001318025.2 | 86.3% | 86% | 116_117ins171 |
| 4 | human | 151987 | PPP4R2 | protein phosphatase 4 regul... | NM_001318027.2 | 58.9% | 54.9% | 0_1ins451;41_42ins62 |
| 5 | mouse | 232314 | Ppp4r2 | protein phosphatase 4, regu... | NM_182939.4 | 85.3% | 81.9% | (many diffs) |
| 6 | mouse | 232314 | Ppp4r2 | protein phosphatase 4, regu... | XM_006506008.3 | 75.5% | 72.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1320
- ORF length:
- 1251
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggacgtcgag aggctccagg aggcgctgaa agattttgag aagaggggga 121 aaaaggaagt ttgtcctgtc ctggatcagt ttctttgtca tgtagccaag actggagaaa 181 caatgattca gtggtcccaa tttaaaggct attttatttt caaactggag aaagtgatgg 241 atgatttcag aacttcagct cctgagccaa gaggtcctcc caaccctaat gtcgaatata 301 ttccctttga tgaaatgaag gaaagaatac tgaaaattgt cactggattt aatggtatcc 361 cttttactat tcagcgacta tgtgaattgt taacagatcc aaggagaaac tatacaggaa 421 cagacaaatt tctcagagga gtagaaaaga atgtgatggt tgttagctgt gtttatcctt 481 cttcagagaa aaacaattcc aatagtttaa atcgaatgaa tggtgttatg tttcctggaa 541 attcaccaag ctatactgag aggtctaata taaatgggcc tgggacaccc aggccactta 601 atcgaccaaa ggtttctttg tcagccccca tgacaacaaa tgggttgcct gagagcacag 661 acagcaaaga ggcaaatttg cagcaaaatg aggagaaaaa tcacagtgac tcttcgacct 721 ctgaatcaga agtttcctca gtgagccctt tgaaaaataa acatccagat gaagatgctg 781 tggaagctga ggggcatgag gtaaaaagac tcaggtttga caaagaaggt gaagtcagag 841 aaacagccag tcaaacgact tccagcgaaa tttcttcagt tatGGTAGGA GAAACAGAAG 901 CATCATCTTC ATCTCAGGAT AAAGACAAAG ATAGCCGTTG TACCCGGCAG CACTGTACAG 961 AAGAGGATGA AGAAGAGGAT GAAGAGGAAG AAGAAGAGTC TTTTATGACA TCAAGAGAAA 1021 TGATCCCAGA AAGAAAAAAT CAAGAAAAAG AATCTGATGA TGCCTTAACT GTGAATGAAG 1081 AGACTTCTGA GGAAAATAAT CAAATGGAGG AATCTGATGT GTCTCAAGCT GAGAAAGATT 1141 TGCTACATTC TGAAGGTAGT GAAAACGAAG GCCCTGTAAG TAGTAGTTCT TCTGACTGCC 1201 GTGAAACAGA AGAATTAGTA GGATCCAATT CCAGTAAAAC TGGAGAGATT CTTTCAGAAT 1261 CATCCATGGA AAATGATGAC GAAGCCACAG AAGTCACCGA TGAACCAATG GAACAAGACT 1321 TGCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 1381 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 1441 TTTATATATC TTGTGGAAAG GACGAGTGGC CATGGTCTTC GTCTAACTTA CGCGTTAAGT 1501 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt