Construct: ORF TRCN0000477614
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000233.1_s317c1
- Derived from:
- ccsbBroadEn_00895
- DNA Barcode:
- GCGAACACCGCTCTGTTCGCTCTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KCNJ10 (3766)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477614
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3766 | KCNJ10 | potassium inwardly rectifyi... | NM_002241.5 | 100% | 100% | |
| 2 | mouse | 16513 | Kcnj10 | potassium inwardly-rectifyi... | NM_001039484.1 | 89.6% | 99.2% | (many diffs) |
| 3 | mouse | 16513 | Kcnj10 | potassium inwardly-rectifyi... | XM_006496677.3 | 89.6% | 99.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1206
- ORF length:
- 1137
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gacgtcagtt gccaaggtgt attacagtca gaccactcag acagaaagcc 121 ggcccctaat gggcccaggg atacgacggc ggagagtcct gacaaaagat ggtcgcagca 181 acgtgagaat ggagcacatt gccgacaagc gcttcctcta cctcaaggac ctgtggacaa 241 ccttcattga catgcagtgg cgctacaagc ttctgctctt ctctgcgacc tttgcaggca 301 catggttcct ctttggcgtg gtgtggtatc tggtagctgt ggcacatggg gacctgctgg 361 agctggaccc cccggccaac cacaccccct gtgtggtaca ggtgcacaca ctcactggag 421 ccttcctctt ctcccttgaa tcccaaacca ccattggcta tggcttccgc tacatcagtg 481 aggaatgtcc actggccatt gtgcttctta ttgcccagct ggtgctcacc accatcctgg 541 aaatcttcat cacaggtacc ttcctggcga agattgcccg gcccaagaag cgggctgaga 601 ccattcgttt cagccagcat gcagttgtgg cctcccacaa tggcaagccc tgcctcatga 661 tccgagttgc caatatgcgc aaaagcctcc tcattggctg ccaggtgaca ggaaaactgc 721 ttcagaccca ccaaaccaag gaaggggaga acatccggct caaccaggtc aatgtgactt 781 tccaagtaga cacagcctct gacagcccct tccttattct accccttacc ttctatcatg 841 tggtagatga gaccagtccc ttgaaagatc tccctcttcg cagtggtgag ggtgactttg 901 agctggtgct gatcctaagt gggacagtgg agtccaccag tgccacctgt CAGGTGCGCA 961 CTTCCTACCT GCCAGAGGAG ATCCTTTGGG GCTACGAGTT CACACCTGCC ATCTCACTGT 1021 CAGCCAGTGG TAAATACATA GCTGACTTTA GCCTTTTTGA CCAAGTTGTG AAAGTGGCCT 1081 CTCCTAGTGG CCTCCGTGAC AGCACTGTAC GCTACGGAGA CCCTGAAAAG CTCAAGTTGG 1141 AGGAGTCATT AAGGGAGCAA GCTGAGAAGG AGGGCAGTGC CCTTAGTGTG CGCATCAGCA 1201 ATGTCTTGCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 1261 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 1321 CTTGGCTTTA TATATCTTGT GGAAAGGACG AGCGAACACC GCTCTGTTCG CTCTCACGCG 1381 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt