Construct: ORF TRCN0000477619
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018161.1_s317c1
- Derived from:
- ccsbBroadEn_11742
- DNA Barcode:
- CTTGATCTACTGTATACCAACAAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TMEM131 (23505)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477619
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23505 | TMEM131 | transmembrane protein 131 | XM_011510869.1 | 13.9% | 13.9% | (many diffs) |
| 2 | human | 23505 | TMEM131 | transmembrane protein 131 | XM_005263912.3 | 13.8% | 13.9% | (many diffs) |
| 3 | human | 23505 | TMEM131 | transmembrane protein 131 | XM_005263911.1 | 13.7% | 13.8% | (many diffs) |
| 4 | human | 23505 | TMEM131 | transmembrane protein 131 | NM_015348.2 | 13.5% | 13.6% | (many diffs) |
| 5 | human | 23505 | TMEM131 | transmembrane protein 131 | XM_005263909.1 | 13.4% | 13.5% | (many diffs) |
| 6 | human | 23505 | TMEM131 | transmembrane protein 131 | XM_005263910.1 | 13.3% | 13.4% | (many diffs) |
| 7 | human | 23505 | TMEM131 | transmembrane protein 131 | XR_001738682.1 | 10.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 858
- ORF length:
- 789
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gagttctctc tttgcttctt tttcaagtga ccctaaaata aaacagccaa 121 atggaagcaa acacaagttg acaaaggcag cctcgctccc gggcaagaac ggcaacccca 181 cttttgctgc agtcacggct ggctacgaca agagcccagg tgggaatggc tttgctaaag 241 tttcttcaaa caaaacaggt ttctccagca gccttggcat ttcacacgct cctgttgaca 301 gcgatggctc agacagctcg ggtttgtgga gtcccgtcag caacccaagc agccctgact 361 tcactcccct caattcgttc tccgcctttg gaaactcttt taatctaact ggtgaagttt 421 tcagcaaact cggattatct cgatcgtgca atcaggccTC ACAGAGGAGC TGGAACGAGT 481 TTAATAGTGG CCCTTCATAC CTTTGGGAGT CGCCAGCGAC AGATCCCAGT CCTTCCTGGC 541 CAGCCAGTTC CGGCTCCCCG ACCCACACAG CCACATCGGT CCTCGGTAAC ACCAGCGGCC 601 TGTGGTCCAC CACTCCATTC AGCAGCTCCA TTTGGTCCAG CAACCTTAGC AGCGCCCTTC 661 CCTTCACCAC TCCAGCAAAC ACGCTGGCAA GCATCGGCCT CATGGGCACA GAAAACTCCC 721 CTGCTCCTCA CGCTCCCTCC ACCTCCAGTC CAGCTGACGA CTTGGGACAG ACCTACAACC 781 CGTGGCGGAT ATGGAGCCCC ACGATTGGAA GAAGAAGCTC GGACCCTTGG TCTAATTCGC 841 ACTTTCCTCA CGAGAATTTG CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 901 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 961 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGACTTGATC TACTGTATAC 1021 CAACAACACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt