Construct: ORF TRCN0000477629
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016164.1_s317c1
- Derived from:
- ccsbBroadEn_02845
- DNA Barcode:
- CACGATGATTCGACTCGATGGTAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PRPF6 (24148)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477629
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 24148 | PRPF6 | pre-mRNA processing factor 6 | NM_012469.4 | 100% | 100% | |
| 2 | human | 24148 | PRPF6 | pre-mRNA processing factor 6 | XM_006723769.3 | 92.2% | 92.2% | 1303_1304ins219 |
| 3 | mouse | 68879 | Prpf6 | pre-mRNA splicing factor 6 | NM_133701.2 | 88.7% | 98.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2889
- ORF length:
- 2823
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa caagaagaag aaaccgttcc tagggatgcc cgcgcccctc ggctacgtgc 121 cggggctggg ccggggcgcc actggcttca ccacgcggtc agacattggg cccgcccgtg 181 atgcaaatga ccctgtggat gatcgccatg cacccccagg caagagaacc gttggggacc 241 agatgaagaa aaatcaggct gctgacgatg acgacgagga tctaaatgac accaattacg 301 atgagtttaa tggctatgct gggagcctct tctcaagtgg accctacgag aaagatgatg 361 aggaagcaga tgctatctat gcagccctgg ataaaaggat ggatgaaaga agaaaagaaa 421 gacgggagca aagggagaaa gaagaaatag agaaatatcg tatggaacgc cccaaaatcc 481 aacagcagtt ctcagacctc aagaggaagt tggcagaagt cacagaagaa gagtggctga 541 gcatccccga ggttggcgat gccagaaata aacgtcagcg gaacccacgc tatgagaagc 601 tgacccctgt tcctgacagt ttctttgcca aacatttaca gaccggagag aaccatacct 661 cagtggatcc ccgacaaact caatttggag gtcttaacac accctatcca ggtggactaa 721 acactccata cccaggtgga atgacgccag gactgatgac acctggcaca ggtgagctgg 781 acatgaggaa gattggccaa gcgaggaaca ctctgatgga catgaggctg agccaggtgt 841 ctgactccgt gagtggacag accgtcgttg accccaaagg ctacctgacg gatttaaatt 901 ccatgatccc gacacacgga ggagacatca atgatatcaa gaaggcgcga ctgctcctca 961 agtctgttcg ggagacgaac cctcatcacc cgccagcctg gattgcatca gcccgcctgg 1021 aagaagtcac tgggaagcta caagtagctc ggaaccttat catgaagggg acggagatgt 1081 gccccaagag tgaagatgtc tggctggaag cagccaggtt gcagcctggg gacacagcca 1141 aggccgtggt agcccaagct gtccgtcatc tcccacagtc tgtcaggatt tacatcagag 1201 ccgcagagct ggaaacggac attcgtgcaa agaagcgggt tcttcggaaa gccctcgagc 1261 atgttccaaa ctcggttcgc ttgtggaaag cagccgttga gctggaagaa cctgaagatg 1321 ctagaatcat gctgagccga gctgtggagt gctgccccac cagcgtggag ctctggcttg 1381 ctctggcaag gctggagacc tatgaaaatg cccgcaaggt cttgaacaag gcgcgggaga 1441 acattcctac agaccgacat atctggatca cggctgctaa gctggaggaa gccaatggga 1501 acacgcagat ggtggagaag atcatcgacc gagccatcac ctcgctgcgg gccaacggtg 1561 tggagatcaa ccgtgagcag tggatccagg atgccgagga atgtgacagg gctgggagtg 1621 tggccacctg ccaggccgtc atgcgtgccg tgattgggat tgggattgag gaggaagatc 1681 ggaagcatac ctggatggag gatgctgaca gttgtgtagc ccacaatgcc ctggagtgtg 1741 cacgagccat ctacgcctac gccctgcagg tgttccccag caagaagagt gtgtggctgc 1801 gcgccgcgta cttcgagaag aaccatggca ctcgggagtc cctggaagca ctcctgcaga 1861 gggctgtggc ccactgcccc aaagcagagg tgctgtggct catgggcgcc aagtccaagt 1921 ggctggcagg ggatgtgcct gcagcaagga gcatcctggc cctggccttc caggccaacc 1981 ccaacagtga ggagatctgg ctggcagccg tgaagctgga gtccgagaat gatgagtacg 2041 agcgggcccg gaggctgctg gccaaggcgc ggagcagtgc ccccaccgcc cgggtgttca 2101 tgaagtctgt gaagctggag tgggtgcaag acaacatcag ggcagcccaa gatctgtgcg 2161 aggaggccct gcggcactat gaggacttcc ccaagctgtg gatgatgaag gggcagatcg 2221 aggagcagaa ggagatgatg gagaaggcgc gggaagccta taaccagggg ttgaagaagt 2281 gtccccactc cacacccctg tggcttttgc tctctcggct ggaggagaag attgggcagc 2341 ttactcgagc acgggccatt ttggaaaagt ctcgtctgaa gaacccaaag aaccctgggc 2401 tgtggttgga gtccgtgcgg ctggagtacc gtgcggggct gaagaacatc gcaaatacac 2461 tcatggccaa ggcgctgcag gagtgcccca actccggtat cctgtggtct gaggccatct 2521 tcctcgaggc aaggccccag aggaggacca agagcgtgga tgcccTGAAG AAGTGTGAGC 2581 ATGACCCCCA TGTGCTCCTG GCCGTGGCCA AGCTGTTTTG GAGTCAGCGG AAGATCACCA 2641 AGGCCAGGGA GTGGTTCCAC CGCACTGTGA AGATTGACTC GGACCTGGGG GATGCCTGGG 2701 CCTTCTTCTA CAAGTTTGAG CTGCAGCATG GCACTGAGGA GCAGCAGGAG GAGGTGAGGA 2761 AGCGCTGTGA GAGTGCAGAG CCTCGGCATG GGGAGCTGTG GTGCGCCGTG TCCAAGGACA 2821 TCGCCAACTG GCAGAAGAAG ATCGGGGACA TCCTTAGGCT GGTGGCCGGC CGCATCAAGA 2881 ACACCTTCTG CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 2941 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 3001 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGACACGAT GATTCGACTC GATGGTAAAC 3061 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt