Construct: ORF TRCN0000477641
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001125.1_s317c1
- Derived from:
- ccsbBroadEn_13736
- DNA Barcode:
- ATAGGCTGTGTCAGCTCATTACTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GXYLT2 (727936)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477641
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 727936 | GXYLT2 | glucoside xylosyltransferase 2 | XM_011534068.2 | 100% | 100% | |
| 2 | human | 727936 | GXYLT2 | glucoside xylosyltransferase 2 | XM_011534067.3 | 87% | 87% | 1_141del |
| 3 | human | 727936 | GXYLT2 | glucoside xylosyltransferase 2 | NM_001080393.2 | 71.5% | 71.5% | 1_378del |
| 4 | human | 727936 | GXYLT2 | glucoside xylosyltransferase 2 | XM_017007123.2 | 64% | 64% | 1_141del;362_363ins252 |
| 5 | human | 727936 | GXYLT2 | glucoside xylosyltransferase 2 | XM_017007124.2 | 63.7% | 47.1% | 1_141del;613_614ins124;837_838ins131 |
| 6 | human | 727936 | GXYLT2 | glucoside xylosyltransferase 2 | NR_138564.2 | 28.8% | 1_566del;1038_1039insAGAAC;1513_3271del | |
| 7 | mouse | 232313 | Gxylt2 | glucoside xylosyltransferase 2 | NM_198612.2 | 62.3% | 67.1% | (many diffs) |
| 8 | mouse | 232313 | Gxylt2 | glucoside xylosyltransferase 2 | XM_006506007.4 | 53.3% | 57.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1020
- ORF length:
- 951
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatcat gctcaaatca gctgtgcttt ttagccacag gaagatccaa ttccacatct 121 tcactgaaga ctctctgaag cccgagtttg ataagcagtt acgccagtgg cctgactcat 181 atacaaagaa gtttgagcac agaatctacc ccatcacatt ttctgttgga aaccctcagg 241 agtggaagaa attgttcaaa ccctgtgctg cccagagact ctttcttccg gtgattttaa 301 aggatgtgga ctcacttctc tacgtggaca ccgatgtcct ctttctgaga cctgttgatg 361 acatctggaa gcttctgagg ctgtttaatt ccacccagct tgcagccatg gcccctgagc 421 acgaaatccc caagattggc tggtacagcc gctttgctag gcatcctttc tatggctctg 481 caggagttaa ttcaggagtc atgttaatga atttaactcg gataagaagt acccagttca 541 agaacagcat gattccaaca ggcctggctt gggaggacat gttgtacccT CTGTACCAGA 601 AGTACAAGAA TGCCATCACG TGGGGAGACC AGGATTTATT AAATATTATT TTTTATTTCA 661 ACCCAGAGTG TCTCTATGTA TTCCCCTGCC AGTGGAACTA CCGTCCCGAT CACTGCATGT 721 ACGGAAGCAA CTGCAGAGAG GCTGAGCATG AAGGTGTGTC TGTTCTGCAT GGAAACCGAG 781 GCGTCTACCA TGACGATAAG CAACCAACGT TCAGAGCACT CTATGAAGCA ATACGGGATT 841 TTCCCTTTCA AGACAATCTC TTTCAATCCA TGTATTACCC CCTTCAGCTG AAGTTTTTGG 901 AGACTGTGCA CACTTTATGT GGACGAATCC CGCAAGTTTT TCTGAAGCAA ATTGAGAAAA 961 CAATGAAAAG GGCTTATGAG AAACACGTCA TCATCCATGT TGGCCCCAAC CAGATGCACT 1021 TGCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 1081 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 1141 TTTATATATC TTGTGGAAAG GACGAATAGG CTGTGTCAGC TCATTACTTA CGCGTTAAGT 1201 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt