Construct: ORF TRCN0000477655
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010513.1_s317c1
- Derived from:
- ccsbBroadEn_09769
- DNA Barcode:
- TGTTGCTGTTGCAAATTAATAGAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FAM151B (167555)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477655
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 167555 | FAM151B | family with sequence simila... | NM_205548.3 | 99.8% | 99.6% | 23C>A |
| 2 | human | 167555 | FAM151B | family with sequence simila... | XM_006714564.3 | 98.5% | 97.1% | (many diffs) |
| 3 | human | 167555 | FAM151B | family with sequence simila... | XM_011543234.2 | 93.6% | 92.4% | (many diffs) |
| 4 | human | 167555 | FAM151B | family with sequence simila... | XM_017009166.1 | 87.7% | 80% | (many diffs) |
| 5 | human | 167555 | FAM151B | family with sequence simila... | XM_017009167.1 | 87.7% | 80% | (many diffs) |
| 6 | human | 167555 | FAM151B | family with sequence simila... | XM_011543235.2 | 84.6% | 84.4% | 23C>A;25_26ins126 |
| 7 | human | 167555 | FAM151B | family with sequence simila... | XM_011543236.2 | 80.4% | 80.4% | 0_1ins162 |
| 8 | human | 167555 | FAM151B | family with sequence simila... | XM_017009168.1 | 80.4% | 80.4% | 0_1ins162 |
| 9 | human | 167555 | FAM151B | family with sequence simila... | XM_017009169.1 | 80.4% | 80.4% | 0_1ins162 |
| 10 | human | 167555 | FAM151B | family with sequence simila... | XM_011543237.2 | 62.8% | 61.9% | (many diffs) |
| 11 | human | 167555 | FAM151B | family with sequence simila... | XM_017009170.1 | 59% | 59% | 0_1ins339 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 897
- ORF length:
- 828
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcagcatcc gctggaggcc aaggatcttg gagtgaaaat atactggaat 121 attttctgag aaatagccag attacagcag aagacggtgc tgagatcacc tggtatcatg 181 cagctaacca caaggcacaa acaaatgagg cactgaaaag tactgctcac atgatagagg 241 ctgatgtcct tcttccaagt gatggatcag aacacagcca gccaattatg gcccatcccc 301 ctgaaacaaa cagtgataat actctacagg agtggctgac tgaagttatg aaaagcaata 361 aaggcatcaa gctggatttc aaaagtctgg cagttgtaga accatccatg atgctcttgg 421 aaaatgtgaa gaggcatctg aagcgtcctg tatggattaa tgccgatatt cttcctggtc 481 caaatggaaa tagcaaagta atagatgcaa aaccattttt agacaccgtg atatccttct 541 ttccagacgt gacgttttcc cTGGGTTGGA CAACAGGATG GCATCCTGAG AAAGTCAATG 601 AAGGGTACAG TTGGACAATG GTGAAAGAGA TGGAATATAT ATGTAATGAA CTAAGTCAGC 661 CTGTAACGTT CCCTGTCAGA GCAGCATTAG TCAGGCAGTC TTGTTCTCAG TTACTTTGGC 721 TGTTAAAGAA ATCAAACAGG TACAGCCTGA CTATTTGGAC TGGAAAAAAT GATAACTATT 781 CCGTTGAAGA TTTACTTTAC ATTAGAGACC ATTTTGACAA AAAACAAGTT TTCTATGACA 841 TCTTGGAACC ACAAAACCAT GAATTTAAAC AAGCCATTGG AATCAAAGTT AATCTCTTGC 901 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 961 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 1021 ATATATCTTG TGGAAAGGAC GATGTTGCTG TTGCAAATTA ATAGATACGC GTTAAGTCga 1081 caatcaacct ctggattaca aaatttgtga aagatt