Construct: ORF TRCN0000477661

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF017917.1_s317c1
Derived from:
ccsbBroadEn_04970
DNA Barcode:
CCTTAGCGTGTTAGTGTGTTGAGC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
ALG10B (144245)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000477661
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 144245 ALG10B ALG10 alpha-1,2-glucosyltra... NM_001013620.4 99.9% 99.7% 251C>G
2 human 84920 ALG10 ALG10 alpha-1,2-glucosyltra... NM_032834.4 97.8% 96.1% (many diffs)
3 human 144245 ALG10B ALG10 alpha-1,2-glucosyltra... XM_005268665.4 87.2% 87.1% 0_1ins180;71C>G
4 human 84920 ALG10 ALG10 alpha-1,2-glucosyltra... XM_024449230.1 85.4% 83.7% (many diffs)
5 human 84920 ALG10 ALG10 alpha-1,2-glucosyltra... XM_024449231.1 85.4% 83.7% (many diffs)
6 human 144245 ALG10B ALG10 alpha-1,2-glucosyltra... NM_001308340.2 26.1% 25.7% (many diffs)
7 mouse 380959 Alg10b asparagine-linked glycosyla... NM_001033441.3 82.4% 85.2% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1488
ORF length:
1419
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat ggcgcagcta gagggttact gtttctcggc cgccttgagc tgtacctttt
121tagtgtcctg cctcctcttc tccgccttca gccgggcgct gcgagagccc tacatggacg
181agatcttcca cctgcctcag gcgcagcgct actgtgaggg ccatttctcc ctttcccagt
241gggatcccat gattactaca ttacctggct tgtacctggt gtcagttgga gtggtcaaac
301ctgccatttg gatctttgga tggtctgaac atgttgtctg ctccattggg atgctcagat
361ttgttaatct tctcttcagt gttggcaact tctatttact atatttgctt ttccacaagg
421tacaacccag aaacaaggct gcctcaagta tccagagagt cttgtcaaca ttaacactag
481cagtatttcc aacactttat ttttttaact tcctttatta tacagaagca ggatctatgt
541tttttactct ttttgcatat ttgatgtgtc tttatggaaa tcataaaact tcagccttcc
601ttggattttg tggcttcatg tttcggcaaa caaatatcat ctgggctgtc ttctgtgcag
661ggaatgtcat tgcacaaaag ttaactgagg cttggaaaac tgagctacaa aagaaggaag
721acagacttcc acctattaaa ggaccatttg cagaattcag aaaaattctt cagtttcttt
781tggcttattc catgtccttt aaaaacttga gtatgctttt ctgtttgact tggccctaca
841tccttctggg atttctgttt tgtgcttttg tagtagttaa tggtggaatt gttattggcg
901atcggagtag tcatgaagcc tgtcttcatt ttcctcaact attctacttt ttttcattta
961ctctcttttt ttcttttcct catctcctgt ctcctagcaa aattaagact tttctttcct
1021tagtttggaa acatggaaTT CTGTTTTTGG TGGTTACCTT AGTCTCTGTG TTTTTAGTTT
1081GGAAATTCAC TTATGCTCAT AAATACTTGC TAGCAGACAA TAGACATTAT ACTTTCTATG
1141TGTGGAAAAG AGTTTTTCAA AGATATGCAA TTCTGAAATA TTTGTTAGTT CCAGCCTATA
1201TATTTGCTGG TTGGAGTATA GCTGACTCAT TGAAATCAAA GCCAATTTTT TGGAATTTAA
1261TGTTTTTCAT ATGCTTGTTC ATTGTTATAG TTCCTCAGAA ACTGCTGGAA TTTCGTTACT
1321TCATTTTACC TTATGTCATT TATAGGCTTA ACATAACTCT GCCTCCCACA TCCAGACTTG
1381TTTGTGAACT GAGTTGCTAT GCAATTGTTA ATTTCATAAC TTTTTACATC TTTCTGAACA
1441AGACTTTTCA GTGGCCAAAT AGTCAGGACA TTCAAAGGTT TATGTGGTTG CCAACTTTCT
1501TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA
1561CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT
1621GTGGAAAGGA CGACCTTAGC GTGTTAGTGT GTTGAGCACG CGTTAAGTCg acaatcaacc
1681tctggattac aaaatttgtg aaagatt

Download FASTA (ORF) (Full)

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