Construct: ORF TRCN0000477661
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017917.1_s317c1
- Derived from:
- ccsbBroadEn_04970
- DNA Barcode:
- CCTTAGCGTGTTAGTGTGTTGAGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ALG10B (144245)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477661
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 144245 | ALG10B | ALG10 alpha-1,2-glucosyltra... | NM_001013620.4 | 99.9% | 99.7% | 251C>G |
| 2 | human | 84920 | ALG10 | ALG10 alpha-1,2-glucosyltra... | NM_032834.4 | 97.8% | 96.1% | (many diffs) |
| 3 | human | 144245 | ALG10B | ALG10 alpha-1,2-glucosyltra... | XM_005268665.4 | 87.2% | 87.1% | 0_1ins180;71C>G |
| 4 | human | 84920 | ALG10 | ALG10 alpha-1,2-glucosyltra... | XM_024449230.1 | 85.4% | 83.7% | (many diffs) |
| 5 | human | 84920 | ALG10 | ALG10 alpha-1,2-glucosyltra... | XM_024449231.1 | 85.4% | 83.7% | (many diffs) |
| 6 | human | 144245 | ALG10B | ALG10 alpha-1,2-glucosyltra... | NM_001308340.2 | 26.1% | 25.7% | (many diffs) |
| 7 | mouse | 380959 | Alg10b | asparagine-linked glycosyla... | NM_001033441.3 | 82.4% | 85.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1488
- ORF length:
- 1419
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcgcagcta gagggttact gtttctcggc cgccttgagc tgtacctttt 121 tagtgtcctg cctcctcttc tccgccttca gccgggcgct gcgagagccc tacatggacg 181 agatcttcca cctgcctcag gcgcagcgct actgtgaggg ccatttctcc ctttcccagt 241 gggatcccat gattactaca ttacctggct tgtacctggt gtcagttgga gtggtcaaac 301 ctgccatttg gatctttgga tggtctgaac atgttgtctg ctccattggg atgctcagat 361 ttgttaatct tctcttcagt gttggcaact tctatttact atatttgctt ttccacaagg 421 tacaacccag aaacaaggct gcctcaagta tccagagagt cttgtcaaca ttaacactag 481 cagtatttcc aacactttat ttttttaact tcctttatta tacagaagca ggatctatgt 541 tttttactct ttttgcatat ttgatgtgtc tttatggaaa tcataaaact tcagccttcc 601 ttggattttg tggcttcatg tttcggcaaa caaatatcat ctgggctgtc ttctgtgcag 661 ggaatgtcat tgcacaaaag ttaactgagg cttggaaaac tgagctacaa aagaaggaag 721 acagacttcc acctattaaa ggaccatttg cagaattcag aaaaattctt cagtttcttt 781 tggcttattc catgtccttt aaaaacttga gtatgctttt ctgtttgact tggccctaca 841 tccttctggg atttctgttt tgtgcttttg tagtagttaa tggtggaatt gttattggcg 901 atcggagtag tcatgaagcc tgtcttcatt ttcctcaact attctacttt ttttcattta 961 ctctcttttt ttcttttcct catctcctgt ctcctagcaa aattaagact tttctttcct 1021 tagtttggaa acatggaaTT CTGTTTTTGG TGGTTACCTT AGTCTCTGTG TTTTTAGTTT 1081 GGAAATTCAC TTATGCTCAT AAATACTTGC TAGCAGACAA TAGACATTAT ACTTTCTATG 1141 TGTGGAAAAG AGTTTTTCAA AGATATGCAA TTCTGAAATA TTTGTTAGTT CCAGCCTATA 1201 TATTTGCTGG TTGGAGTATA GCTGACTCAT TGAAATCAAA GCCAATTTTT TGGAATTTAA 1261 TGTTTTTCAT ATGCTTGTTC ATTGTTATAG TTCCTCAGAA ACTGCTGGAA TTTCGTTACT 1321 TCATTTTACC TTATGTCATT TATAGGCTTA ACATAACTCT GCCTCCCACA TCCAGACTTG 1381 TTTGTGAACT GAGTTGCTAT GCAATTGTTA ATTTCATAAC TTTTTACATC TTTCTGAACA 1441 AGACTTTTCA GTGGCCAAAT AGTCAGGACA TTCAAAGGTT TATGTGGTTG CCAACTTTCT 1501 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 1561 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1621 GTGGAAAGGA CGACCTTAGC GTGTTAGTGT GTTGAGCACG CGTTAAGTCg acaatcaacc 1681 tctggattac aaaatttgtg aaagatt