Construct: ORF TRCN0000477664
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002891.1_s317c1
- Derived from:
- ccsbBroadEn_07383
- DNA Barcode:
- TGTCTACAACAACGAAGACTCAGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- STK17A (9263)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477664
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9263 | STK17A | serine/threonine kinase 17a | NM_004760.3 | 99.9% | 99.7% | 1084A>G |
| 2 | human | 9263 | STK17A | serine/threonine kinase 17a | XM_017012792.1 | 77.9% | 77.7% | 0_1ins273;811A>G |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1308
- ORF length:
- 1242
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgat ccctttggag aagccaggca gcggcggctc ctccccaggc gccacctcag 121 gctcgggccg ggcaggccgg ggtctgagcg ggccgtgccg gccgccgccg ccgccccagg 181 cccgcgggct gctgacagag atacgcgccg tggtgcgcac cgagcccttc caggacggct 241 acagcctgtg cccgggccgg gagctgggca gggggaaatt tgcagtggtg agaaaatgta 301 taaagaaaga ttctgggaaa gaatttgctg caaagttcat gagaaaaaga agaaaaggcc 361 aagattgtcg gatggaaata attcatgaga ttgctgtact tgaactagca caagacaatc 421 cttgggtcat taatttacat gaagtttatg agactgcatc agaaatgatc ttagttctgg 481 aatatgctgc tgggggtgaa atctttgacc agtgtgttgc agacagagaa gaagccttta 541 aagaaaaaga tgttcaaaga cttatgcgac agattttaga aggtgttcac tttttacaca 601 ctcgtgatgt agttcatctt gatttgaagc ctcagaatat tctgttgaca agtgaatctc 661 cattgggtga cattaagatt gttgattttg gcctttcaag aatattgaag aacagtgaag 721 agctccgaga aattatgggt acccctgaat atgtggctcc tgaaattctt agttatgatc 781 ctataagcat ggcaacagat atgtggagca ttggagtgtt aacatatgtc atgcttacag 841 gaatatcacc tttcttaggc aatgataaac aagaaacatt cttaaacatc tcacagatga 901 atttaagtta ttctgaggaa gaatttgatg ttttgtctga gtcggctgtt gatttcaTCA 961 GGACACTTTT AGTTAAGAAA CCTGAAGATC GAGCCACTGC TGAAGAATGT CTAAAGCACC 1021 CCTGGTTGAC ACAGAGCAGT ATTCAAGAGC CTTCTTTCAG GATGGAAAAG GCACTAGAAG 1081 AAGCAAATGC CCTCCAAGAA GGTCATTCTG TGCCTGAAAT TAATTCGGAT ACCGACAAAT 1141 CAGAAACCGA GGAATCCATT GTAACCGAAG AGTTAATTGT AGTTACTTCA TATACTCTAG 1201 GACAATGCAG ACAGTCTGAA AAAGAGAAAA TGGAGCAAAA GGCCATTTCC AAACGATTTA 1261 AATTTGAGGA ACCTTTGCTA CAAGAAATTC CAGGAGAATT TATCTACTGC CCAACTTTCT 1321 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 1381 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1441 GTGGAAAGGA CGATGTCTAC AACAACGAAG ACTCAGCACG CGTTAAGTCg acaatcaacc 1501 tctggattac aaaatttgtg aaagatt