Transcript: Human NM_004760.3

Homo sapiens serine/threonine kinase 17a (STK17A), mRNA.

Source:
NCBI, updated 2019-08-07
Taxon:
Homo sapiens (human)
Gene:
STK17A (9263)
Length:
3918
CDS:
137..1381

Additional Resources:

NCBI RefSeq record:
NM_004760.3
NBCI Gene record:
STK17A (9263)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001487063 CTTCTCTGTCTGCAACACAC pXPR_003 TGG 449 36% 3 0.4429 STK17A STK17A 77915
2 BRDN0001147331 AAAAGGCCAAGATTGTCGGA pXPR_003 TGG 304 24% 2 0.2486 STK17A STK17A 77918
3 BRDN0001149521 TTCTAGGGGGAAATTTGCAG pXPR_003 TGG 217 17% 2 0.0226 STK17A STK17A 77917
4 BRDN0001149480 GCTGACAGAGATACGCGCCG pXPR_003 TGG 142 11% 1 -0.1846 STK17A STK17A 77916
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_004760.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000379507 TCTTAGTTATGATCCTATAAG pLKO_005 838 CDS 100% 13.200 18.480 N STK17A n/a
2 TRCN0000194941 CTATTGAGTTTACTCGGTTTA pLKO.1 3629 3UTR 100% 10.800 15.120 N STK17A n/a
3 TRCN0000000974 CCTATAAGCATGGCAACAGAT pLKO.1 851 CDS 100% 4.950 6.930 N STK17A n/a
4 TRCN0000380630 GACAATCCTTGGGTCATTAAT pLKO_005 485 CDS 100% 15.000 10.500 N STK17A n/a
5 TRCN0000272431 GGCCATTTCCAAACGATTTAA pLKO_005 1312 CDS 100% 15.000 10.500 N STK17A n/a
6 TRCN0000379434 AGTTACCCGTTTCAATGTTAT pLKO_005 1579 3UTR 100% 13.200 9.240 N STK17A n/a
7 TRCN0000010569 TGTGGAGCATTGGAGTGTTAA pLKO.1 873 CDS 100% 13.200 9.240 N STK17A n/a
8 TRCN0000272512 TGGATAACCAGTATCACTTAC pLKO_005 1473 3UTR 100% 10.800 7.560 N STK17A n/a
9 TRCN0000000976 CAGAGCAGTATTCAAGAGCCT pLKO.1 1103 CDS 100% 0.660 0.462 N STK17A n/a
10 TRCN0000272485 CAGAGCAGTATTCAAGAGCCT pLKO_005 1103 CDS 100% 0.660 0.462 N STK17A n/a
11 TRCN0000000973 CTAGTAAGAGAGGTGGTAATT pLKO.1 2017 3UTR 100% 0.000 0.000 N STK17A n/a
12 TRCN0000284763 CAGTGAAGAGCTCCGAGAAAT pLKO_005 784 CDS 100% 13.200 7.920 N STK17A n/a
13 TRCN0000000975 GAGATTGCTGTACTTGAACTA pLKO.1 458 CDS 100% 4.950 2.970 N STK17A n/a
14 TRCN0000272430 GAGATTGCTGTACTTGAACTA pLKO_005 458 CDS 100% 4.950 2.970 N STK17A n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_004760.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_07383 pDONR223 100% 99.9% 99.7% None 1084A>G n/a
2 ccsbBroad304_07383 pLX_304 0% 99.9% 99.7% V5 1084A>G n/a
3 TRCN0000477664 TGTCTACAACAACGAAGACTCAGC pLX_317 28.6% 99.9% 99.7% V5 1084A>G n/a
4 TRCN0000487751 TGCCCTGACGACATTAAAACGATC pLX_317 13% 99.9% 99.7% V5 (not translated due to prior stop codon) 1084A>G n/a
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