Construct: ORF TRCN0000477667
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017869.1_s317c1
- Derived from:
- ccsbBroadEn_05926
- DNA Barcode:
- CGGTCGTCAAAGCTATTCCAACTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CACNG1 (786)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477667
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 786 | CACNG1 | calcium voltage-gated chann... | NM_000727.4 | 99.8% | 100% | 273C>T |
| 2 | mouse | 12299 | Cacng1 | calcium channel, voltage-de... | NM_007582.2 | 80.8% | 85.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 735
- ORF length:
- 666
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gtcccagacc aaaatgctga aggtccgcgt gaccctcttc tgcatcctgg 121 caggcatcgt gctggccatg acagccgtgg taaccgacca ctgggctgtg ctgagccccc 181 acatggagca ccacaacact acctgcgagg cggcccactt cggcctctgg cggatttgta 241 ccaagcgcat ccccatggac gacagcaaga cctgcgggcc catcaccctg cccggggaga 301 agaactgttc ctacttcagg cattttaacc ccggcgagag ttcggagatc ttcgaattca 361 ccactcagaa ggagtacagc atctcggcag ccgccatcgc catcttcagc cttggcttca 421 TCATCCTGGG CAGCCTCTGT GTCCTCCTGT CCCTCGGGAA GAAGAGGGAC TATCTGCTGC 481 GACCCGCGTC CATGTTCTAT GCCTTTGCAG GTCTCTGCAT CCTCGTCTCG GTGGAGGTCA 541 TGCGGCAGTC GGTGAAGCGC ATGATTGACA GTGAGGACAC CGTCTGGATC GAGTACTATT 601 ACTCCTGGTC CTTTGCCTGC GCCTGTGCCG CCTTCATCCT CCTCTTTCTC GGCGGTCTCG 661 CCCTCCTGCT GTTCTCCCTG CCTCGAATGC CCCGGAACCC ATGGGAGTCC TGCATGGATG 721 CTGAGCCCGA GCACTTGCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 781 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 841 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA CGGTCGTCAA AGCTATTCCA 901 ACTAACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt