Construct: ORF TRCN0000477667

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF017869.1_s317c1
Derived from:
ccsbBroadEn_05926
DNA Barcode:
CGGTCGTCAAAGCTATTCCAACTA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
CACNG1 (786)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000477667
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 786 CACNG1 calcium voltage-gated chann... NM_000727.4 99.8% 100% 273C>T
2 mouse 12299 Cacng1 calcium channel, voltage-de... NM_007582.2 80.8% 85.2% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
735
ORF length:
666
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat gtcccagacc aaaatgctga aggtccgcgt gaccctcttc tgcatcctgg
121caggcatcgt gctggccatg acagccgtgg taaccgacca ctgggctgtg ctgagccccc
181acatggagca ccacaacact acctgcgagg cggcccactt cggcctctgg cggatttgta
241ccaagcgcat ccccatggac gacagcaaga cctgcgggcc catcaccctg cccggggaga
301agaactgttc ctacttcagg cattttaacc ccggcgagag ttcggagatc ttcgaattca
361ccactcagaa ggagtacagc atctcggcag ccgccatcgc catcttcagc cttggcttca
421TCATCCTGGG CAGCCTCTGT GTCCTCCTGT CCCTCGGGAA GAAGAGGGAC TATCTGCTGC
481GACCCGCGTC CATGTTCTAT GCCTTTGCAG GTCTCTGCAT CCTCGTCTCG GTGGAGGTCA
541TGCGGCAGTC GGTGAAGCGC ATGATTGACA GTGAGGACAC CGTCTGGATC GAGTACTATT
601ACTCCTGGTC CTTTGCCTGC GCCTGTGCCG CCTTCATCCT CCTCTTTCTC GGCGGTCTCG
661CCCTCCTGCT GTTCTCCCTG CCTCGAATGC CCCGGAACCC ATGGGAGTCC TGCATGGATG
721CTGAGCCCGA GCACTTGCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC
781CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA
841TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA CGGTCGTCAA AGCTATTCCA
901ACTAACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt

Download FASTA (ORF) (Full)