Construct: ORF TRCN0000477683
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009495.1_s317c1
- Derived from:
- ccsbBroadEn_07436
- DNA Barcode:
- CCCGCGACTCTGGTAGTAACTTAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SPTLC2 (9517)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477683
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9517 | SPTLC2 | serine palmitoyltransferase... | NM_004863.3 | 99.9% | 100% | 786T>C |
| 2 | human | 9517 | SPTLC2 | serine palmitoyltransferase... | XM_011537384.2 | 79.6% | 78% | (many diffs) |
| 3 | mouse | 20773 | Sptlc2 | serine palmitoyltransferase... | NM_011479.4 | 90.6% | 96% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1752
- ORF length:
- 1686
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcg gccggagccc ggaggctgct gctgccgccg cacggtgcgg gcgaatggct 121 gcgtggcgaa cggggaagta cggaacgggt acgtgaggag cagcgctgca gccgcagccg 181 cagccgccgc cggccagatc catcatgtta cacaaaatgg aggactatat aaaagaccgt 241 ttaatgaagc ttttgaagaa acaccaatgc tggttgctgt gctcacgtat gtggggtatg 301 gcgtactcac cctctttgga tatcttcgag atttcttgag gtattggaga attgaaaagt 361 gtcaccatgc aacagaaaga gaagaacaaa aggactttgt gtcattgtat caagattttg 421 aaaactttta tacaaggaat ctgtacatga ggataagaga caactggaat cggccaatct 481 gtagtgtgcc tggagccagg gtggacatca tggagagaca gtctcatgat tataactggt 541 ccttcaagta tacagggaat ataataaagg gtgttataaa catgggttcc tacaactatc 601 ttggatttgc acggaatact ggatcatgtc aagaagcagc cgccaaagtc cttgaggagt 661 atggagctgg agtgtgcagt actcggcagg aaattggaaa cctggacaag catgaagaac 721 tagaggagct tgtagcaagg ttcttaggag tagaagctgc tatggcgtat ggcatgggat 781 ttgcaacgaa ttcaatgaac attcctgctc ttgttggcaa aggttgcctg attctgagtg 841 atgaactgaa ccatgcatca ctggttctgg gagccagact gtcaggagca accattagaa 901 tcttcaaaca caacaatatg caaagcctag agaagctatt gaaagatgcc attgtttatg 961 gtcagcctcg gacacgaagg ccctggaaga aaattctcat ccttgtggaa ggaatatata 1021 gcatggaggg atctattgtt cgtcttcctg aagtgattgc cctcaagaag aaatacaagg 1081 catacttgta tctggatgag gctcacagca ttggcgccct gggccccaca ggccggggtg 1141 tggtggagta ctttggcctg gatcccgagg atgtggatgt tatgatggga acgttcacaa 1201 agagttttgg tgcttctgga ggatatattg gaggcaagaa ggagctgata gactacctgc 1261 gaacacattc tcatagtgca gtgtatgcca cgtcattgtc acctcctgta gtggagcaga 1321 tcatcacctc catgaagtgc atcatggggc aggatggcac cagccttggt aaagagtgtg 1381 tacaacagtt agctgaaaac accaggtatt tcaggagacg cctgaaagag atgggcttca 1441 tcatctatgg aaatgaagac tctccagtag tgccttTGAT GCTCTACATG CCTGCCAAAA 1501 TTGGCGCCTT TGGACGGGAG ATGCTGAAGC GGAACATCGG TGTCGTTGTG GTTGGATTTC 1561 CTGCCACCCC AATTATTGAG TCCAGAGCCA GGTTTTGCCT GTCAGCAGCT CATACCAAAG 1621 AAATACTTGA TACTGCTTTA AAGGAGATAG ATGAAGTTGG GGACCTATTG CAGCTGAAGT 1681 ATTCCCGTCA TCGGTTGGTA CCTCTACTGG ACAGGCCCTT TGACGAGACG ACGTATGAAG 1741 AAACAGAAGA CTGCCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 1801 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 1861 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGACCC GCGACTCTGG TAGTAACTTA 1921 GACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t