Construct: ORF TRCN0000477690
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006824.1_s317c1
- Derived from:
- ccsbBroadEn_03700
- DNA Barcode:
- CCGGAGGTACTTTTCTAGTCAATA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PCDHGB2 (56103)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477690
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 56103 | PCDHGB2 | protocadherin gamma subfami... | NM_032096.1 | 100% | 100% | |
| 2 | human | 56103 | PCDHGB2 | protocadherin gamma subfami... | NM_018923.2 | 86.9% | 86.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2502
- ORF length:
- 2433
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gaaagcgagc tcagggaggt gcgggctggt gcggtggctg caggtactgt 121 tgcccttcct gttgtctttg ttccccgggg ctctcccagt ccagatccgc tattcaattc 181 cagaggagct ggccaaaaac tcggtcgtag gaaacctcgc caaggatctg gggctcagcg 241 tccgggactt gccagcccgg aagctgcggg ttagcgcgga gaaggaatat ttcacagtaa 301 acccagaaag cggagactta cttgtgagtg acagaataga ccgagaacag atatgcggga 361 agcagcctct gtgtgttctg gatttcgata ctgtcgctga aaatccacta aatattttct 421 acatagcagt aattgtgcag gatataaatg ataatacccc gctattcaaa cagactaaga 481 ttaatttaaa aattggcgaa tccactaagc caggtacaac atttccactt gacccagccc 541 tggattcaga tgttggtcct aactcactac aaagatacca ccttaatgac aacgagtact 601 ttgatctcgc tgagaaacag actccagatg gtcgtaaata tcctgagttg attctaaaac 661 actctctgga cagagaagag cacagtttac atcaattggt cctcacagct gtggatggcg 721 gagacccacc tcaaagtggc acgacccaaa tccgaatcaa agtcacggat gccaacgata 781 accctccagt gttcagccag gacgtgtaca gggtcaccct gagggaggac gtgccgccgg 841 gcttctttgt gcttcaagtg acagccaccg accgggatga aggcataaac gcagagatca 901 cctactcctt tcataatgtg gacgaacaag tgaaacactt tttcaactta aatgaaaaaa 961 caggagaaat cacgacaaag gatgatttgg attttgagat tgcaagtagt tacactctga 1021 gtatcgaagc aaaagatcct ggagatctag cagcccactg cagtatccaa gttgaaattc 1081 ttgatgacaa cgattgtgca cctgaagtta ttgtgacttc agtatctact cccctaccgg 1141 aggattcgcc accaggaaca gtgatcgcct tgataaaaac gagagacaga gactctggag 1201 aaaatggaga agtttactgc caagtgttgg gaaatgccaa gtttattttg aaatcttcct 1261 caaagaacta ttacaaacta gtgacagacg gcgctctgga ccgggaggag atcccagaat 1321 acaatctcac catcacagcc accgacgggg gcaagccgcc cctctcctcc agcataattg 1381 tcaccctgca catctccgac gtcaacgata atgccccagt tttccaacag acttcctaca 1441 tggttcacgt ggcagagaac aatcctcctg gcgcctctat cgctcaaatc agtgcctctg 1501 accctgactt gggccccagt ggccaagttt cctactccat cgtagcgagc gacctgaagc 1561 cgcgggagat tttatcctac gtgtccgtga gcgcgcagag cggggtggtg ttcgcgcagc 1621 gcgccttcga tcatgagcag ctgcgcgcct tcgagctcac actgcaggcc cgcgaccagg 1681 gctcgcccgc gctcagcgcc aacgtgagcc tgcgcgtgtt agtgggcgac ctcaatgaca 1741 atgcgccacg ggtgctgtac cccgcgctgg ggcctgatgg ctccgccctc ttcgatatgg 1801 tgccacgcgc cgcagagccc ggctacctgg tgaccaaggt ggtggcggtg gacgcagact 1861 caggacacaa cgcttggctg tcctaccacg tgctgcaggc cagcgagccc gggctcttca 1921 gcctggggtt gcgcacgggt gaggtgcgca cagcgcgtgc cttgggcgac agggacgcgg 1981 cccgccagcg cctgctggtc gctgtgcgtg atggaggaca gccgccactc tccgctacgg 2041 ccacgctgca cctaatcttc gcggatagcc tgcaagaggt attgccagac ctcagcgacc 2101 gccgggagcc ctctgacccc caggcaaaac tgcagtttta cctggttgtg gccttggcct 2161 tgatctcagt gctcttcttc ctcgcggtga ttctggcaat ctccctgcgc cTGCGACTCT 2221 CTTCCAGGTC AGATGCTTGG GACTGTTTTC AGCCTGGTCT CAGCTCCAAG CCTGGACCTG 2281 GGGTTCTCCC CAATTACAGT GAGGGTACAT TGCCCTATTC CTACAACCTG TGTGTTGCCT 2341 CACAATCAGC CAAGACAGAG TTCAATTTTC TGAACATAAC CCCGGAATTG GTTCCCGCGC 2401 AAGATCTCGT CTGTGACAAT GCCTCTTGGG AACAAAATAC AAATCATGGA GCCGCTGGGG 2461 TCCCTTTTGC CTCAGATACT ATTTTGAAGG TGAGCTTTAA TTTGCCAACT TTCTTGTACA 2521 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 2581 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 2641 AGGACGACCG GAGGTACTTT TCTAGTCAAT AACGCGTTAA GTCgacaatc aacctctgga 2701 ttacaaaatt tgtgaaagat t