Construct: ORF TRCN0000477696
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007436.1_s317c1
- Derived from:
- ccsbBroadEn_14475
- DNA Barcode:
- TACAGTTTACTCATAATTACGAAT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- ZNF678 (339500)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477696
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 339500 | ZNF678 | zinc finger protein 678 | NM_001367909.1 | 99.1% | 25.5% | (many diffs) |
| 2 | human | 339500 | ZNF678 | zinc finger protein 678 | NM_001367910.1 | 99.1% | 25.5% | (many diffs) |
| 3 | human | 339500 | ZNF678 | zinc finger protein 678 | NM_001367911.1 | 92% | 19.6% | (many diffs) |
| 4 | human | 339500 | ZNF678 | zinc finger protein 678 | NM_178549.4 | 87.2% | 18.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 471
- ORF length:
- 405
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg cacaatatca ctttgcattg gtgtctgtgc atttgaagga gcaaacacct 121 ctaccagttt ttataaactg gtttatacag ctattttatc ttattccatt caagaccttt 181 tgccagagca ggatatgaaa gatttatgcc aaaaagtgac actgacaaga catagaagct 241 ggggccttga caatttgcac ttagtgaaag actggagaac tgtgaatgaa ggtaaggggc 301 agaaagaata ttgcaataga cttactcaat gttcatcaac taaaagcaaa atctttcaat 361 gtattgaatg tggcagaaat tttagctgga ggtcaatcct tactgaacat aagagaattc 421 atactggaga gaagccatac aaatgtgaag aatgtggcaa agtctttcaa tagatgtttc 481 caaacctaac aaaacatgaa aggaattcat actggagaga aaccntacaa atgtgacgaa 541 tgtggcaaag tttttaattg gtggtcacaa ctaactaacc ataagaaaat tcatactgga 601 gagaaaccct acaaatgtga tgaatgtgac aaagttttta attggtggtc acaactaact 661 agccataagn aaattcatag tggagagaaa ccatacccat gtgaagaatg tggcaaagcc 721 tttacccagt tctcaancct tactgaacat aagagaattc atactggaga gaaaccctac 781 aaatgcaaag aatgttgcaa agcctttaac aagttctcaa accttactca acataagaga 841 attcatactg gagagaaacc ttacaagtgt gaagaatgtg gcaacgtttt taatgagtgc 901 tcacacctaa ctagacatag gagaattcat actggagaga aaccctacaa atgtgaagaa 961 tgtggcaaag cctttacaca gtttgcaagc cttactcgtc ataaaagaat tcatactgga 1021 gaaaaaccct accaatgtga agaatgtggc aaaactttta atcggtgttc acacctaagt 1081 agccataaga gaattcatac tggagagaaa ccctacaaat gtgaagaatg tggcagaacc 1141 tttactcaat tctcaaacct cactcagcat aaaagaattc atactggaga gaaaccctac 1201 aaatgcaaag aatgtggcaa agcgtttaac aagttctcaa gccttactca acataggaga 1261 attcatactg gagtgaaaac ccTACAAATG TGAAGAATGT GGGAAAGTTT TTAAACAGTG 1321 CTCTCACCTA ACTAGCCATA AGAGAATTCA TACTGGAGAG AAACCCTACA AATGTAAAGA 1381 ATGTGGCAAA GCTTTTTACC AATCCTCAAT CCTTAGTAAG CATAAGAGAA TTCATACTGA 1441 AGAGAAACCC TACAAATGTG AAGAATGTGG CAAAGCCTTT AACCAGTTCT CAAGCCTTAC 1501 TCGTCATAAA AGAATTCATA CTGGAGAGAA ACGCTACAAA TGTAAAGAAT GTGGAAAAGG 1561 TTTTTACCAA TCCTCAATCC ATAGTAAGTA TAAGAGAATT TATACTGGAG AGGAACCTGA 1621 CAAATGTAAA AATGTGGCAG TCTTTACCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT 1681 AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA 1741 CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA TACAGTTTAC 1801 TCATAATTAC GAATACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa 1861 gatt