Transcript: Human NM_001367911.1

Homo sapiens zinc finger protein 678 (ZNF678), transcript variant 5, mRNA.

Source:
NCBI, updated 2018-12-28
Taxon:
Homo sapiens (human)
Gene:
ZNF678 (339500)
Length:
8422
CDS:
140..1789

Additional Resources:

NCBI RefSeq record:
NM_001367911.1
NBCI Gene record:
ZNF678 (339500)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001367911.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000018168 CCTCAATCCATAGTAAGTATA pLKO.1 1713 CDS 100% 13.200 18.480 N ZNF678 n/a
2 TRCN0000018170 GTCACAACTAACTAACCATAA pLKO.1 706 CDS 100% 10.800 15.120 N ZNF678 n/a
3 TRCN0000018172 CTTATTCCATTCAAGACCTTT pLKO.1 306 CDS 100% 4.950 6.930 N ZNF678 n/a
4 TRCN0000018169 GCTCTCACCTAACTAGCCATA pLKO.1 1461 CDS 100% 4.050 5.670 N ZNF678 n/a
5 TRCN0000018171 GCAAGCCTTACTCGTCATAAA pLKO.1 1127 CDS 100% 13.200 9.240 N ZNF678 n/a
6 TRCN0000428197 AGAATGTGGCAAAGCGTTTAA pLKO_005 1351 CDS 100% 13.200 6.600 Y ZNF678 n/a
7 TRCN0000428574 CTGAAGAGAAACCCTACAAAT pLKO_005 1578 CDS 100% 13.200 6.600 Y ZNF138 n/a
8 TRCN0000096537 CTGGAGAGAAACCCTACAAAT pLKO.1 654 CDS 100% 13.200 6.600 Y Zfp934 n/a
9 TRCN0000235358 CTGGAGAGAAACCCTACAAAT pLKO_005 654 CDS 100% 13.200 6.600 Y 2810408B13Rik n/a
10 TRCN0000244342 CTGGAGAGAAACCCTACAAAT pLKO_005 654 CDS 100% 13.200 6.600 Y EG668616 n/a
11 TRCN0000435505 ACCCTACAAATGTGATGAATG pLKO_005 748 CDS 100% 10.800 5.400 Y ZNF678 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001367911.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_14475 pDONR223 100% 92% 19.6% None (many diffs) n/a
2 ccsbBroad304_14475 pLX_304 0% 92% 19.6% V5 (not translated due to prior stop codon) (many diffs) n/a
3 TRCN0000477696 TACAGTTTACTCATAATTACGAAT pLX_317 23.3% 92% 19.6% V5 (not translated due to prior stop codon) (many diffs) n/a
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