Construct: ORF TRCN0000477703
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002148.1_s317c1
- Derived from:
- ccsbBroadEn_10671
- DNA Barcode:
- TTATTAGGCTATTGAAGAAAGCCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ADH6 (130)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477703
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 130 | ADH6 | alcohol dehydrogenase 6 (cl... | NM_000672.4 | 80.1% | 74% | 828_963del;1022_1104del |
| 2 | human | 130 | ADH6 | alcohol dehydrogenase 6 (cl... | NM_001102470.2 | 78.6% | 72.6% | 828_963del;1022_1125del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 951
- ORF length:
- 885
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag tactacaggc caagtcatca gatgcaaagc agccatactc tggaagcctg 121 gtgcaccatt ttctattgaa gaggtagaag tggccccacc aaaggcaaag gaagttcgca 181 taaaggttgt ggccaccgga ctgtgtggta cagagatgaa agtgttgggg agtaaacact 241 tggacctctt gtatcccacc atcttgggcc atgaaggggc tggaatcgtt gagagtattg 301 gagaaggagt aagcacagtg aaaccaggtg acaaagttat cacactcttt ctgccacagt 361 gtggagaatg tacctcttgc ctgaattctg agggcaattt ttgtatacaa ttcaaacagt 421 caaaaaccca actgatgtct gatggtacca gcaggtttac ctgcaaggga aaatcaatat 481 atcactttgg taataccagc accttctgtg aatacacagt gataaaggaa atctcagttg 541 ccaagattga tgcagtcgct cctctagaga aagtatgcct aattagctgt ggcttttcca 601 ctgggtttGG TGCTGCAATC AATACTGCCA AGGTGACTCC AGGTTCTACC TGTGCTGTGT 661 TTGGCCTGGG AGGAGTCGGC TTGTCTGTTG TCATGGGTTG TAAAGCAGCA GGAGCAGCCA 721 GGATCATTGG AGTGGATGTC AACAAGGAGA AATTTAAGAA GGCACAGGAA TTGGGTGCTA 781 CTGAGTGCCT CAACCCTCAG GACTTAAAGA AACCCATTCA AGAAGTTTTA TTTGATATGA 841 CAGATGCTGG TATAGACTTC TGCTTTGAGG CCATTGGAAA TCTGGACGTT CTGGCTGGAA 901 GAGCAGACAG CACATCCCTA AACTGGTTGC TGATTATATG GCAGAGAAGT TGCCCAACTT 961 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 1021 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 1081 CTTGTGGAAA GGACGATTAT TAGGCTATTG AAGAAAGCCT ACGCGTTAAG TCgacaatca 1141 acctctggat tacaaaattt gtgaaagatt