Construct: ORF TRCN0000477706
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013633.1_s317c1
- Derived from:
- ccsbBroadEn_13318
- DNA Barcode:
- TTATCAAAGCAATCCTGTGTTTAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SP3P (160824)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477706
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6670 | SP3 | Sp3 transcription factor | NM_001017371.5 | 16.5% | 15.7% | (many diffs) |
| 2 | human | 6670 | SP3 | Sp3 transcription factor | NM_001172712.1 | 15.2% | 14.3% | (many diffs) |
| 3 | human | 6670 | SP3 | Sp3 transcription factor | NM_003111.4 | 15.1% | 14.3% | (many diffs) |
| 4 | mouse | 20687 | Sp3 | trans-acting transcription ... | NM_001098425.1 | 15.9% | 15.2% | (many diffs) |
| 5 | mouse | 20687 | Sp3 | trans-acting transcription ... | NM_001018042.3 | 14.5% | 13.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 447
- ORF length:
- 378
- Sequence:
-
1 TCTTCCATTT CAGGTGTCGT GAGGCTAGCA TCGATTGATC AACAAGTTTG TACAAAAAAG 61 TTGGCACCAT GTATTGTGGT AAAAGATTTA CTCGAAGTGA TGAATTACAG AGGCACAGTA 121 GAACACATAC AGGTGAGAAG AAATTTGTTT GTTCAGAATG TTCAAAATGC TTTGTGAGAA 181 GTGACCATCT TGCCGAACAT ACTAAAACAC AGCAGAATAA AAAAGGTATT CACTCTAGCA 241 GTACGGTGCT GGCATCTGTG GAAGCTGTGC AAGACGATAC TTTGATTACT GCAGGAGGAA 301 CAAAACTTAT CCTTGCAAAT ATTCAACAAG CTTCTGTTTC AGGAATAGGA ACGGTTAATA 361 CCTCTGCCAC CAGCAATCCA GATACCCTTA CCAACACTGA AATACCTTTA CGGCTTGTCA 421 CAGTTTCTGG AAATGAGACA ATGGAGTTGC CAACTTTCTT GTACAAAGTG GTTGATATCG 481 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 541 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GATTATCAAA 601 GCAATCCTGT GTTTATACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 661 aagatt