Construct: ORF TRCN0000477733
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002814.1_s317c1
- Derived from:
- ccsbBroadEn_02342
- DNA Barcode:
- GTAAGCTAGAACCTTCTCAAGCCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RBM7 (10179)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477733
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10179 | RBM7 | RNA binding motif protein 7 | NM_016090.3 | 100% | 100% | |
| 2 | human | 10179 | RBM7 | RNA binding motif protein 7 | NM_001286045.1 | 99.6% | 99.6% | 343_345delAGC |
| 3 | human | 10179 | RBM7 | RNA binding motif protein 7 | NM_001286046.2 | 54.8% | 54.8% | 0_1ins360 |
| 4 | human | 10179 | RBM7 | RNA binding motif protein 7 | NM_001286047.1 | 54.8% | 54.8% | 0_1ins360 |
| 5 | human | 10179 | RBM7 | RNA binding motif protein 7 | NM_001286048.2 | 54.8% | 54.8% | 0_1ins360 |
| 6 | mouse | 67010 | Rbm7 | RNA binding motif protein 7 | NM_144948.5 | 84.8% | 83.4% | (many diffs) |
| 7 | mouse | 67010 | Rbm7 | RNA binding motif protein 7 | NM_001326356.1 | 37.8% | 40.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 864
- ORF length:
- 798
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg ggcggcggcg gcggaagcgg atcgcactct ctttgtgggc aaccttgaaa 121 cgaaagtgac cgaggagctc cttttcgagc ttttccacca ggctgggcca gtaataaagg 181 tgaaaattcc aaaagataag gatggtaaac caaagcagtt tgcgtttgtg aatttcaaac 241 atgaagtgtc tgttccttat gcaatgaatc tacttaatgg aatcaaactt tatggaaggc 301 ctatcaaaat tcaatttaga tcaggaagta gtcatgcccc acaagatgtc agtttgtcat 361 atccccaaca tcatgttGGA AATTCAAGCC CTACCTCCAC ATCTCCTAGC AGGTACGAAA 421 GGACTATGGA TAACATGACT TCATCAGCAC AGATAATTCA GAGATCTTTC TCTTCTCCAG 481 AAAATTTTCA GAGACAAGCA GTGATGAACA GTGCTTTGAG ACAAATGTCA TATGGTGGAA 541 AATTTGGTTC TTCACCTCTG GATCAATCAG GATTTTCACC ATCAGTTCAA TCACACAGTC 601 ATAGTTTCAA TCAGTCTTCA AGCTCCCAGT GGCGCCAAGG TACACCATCA TCACAGCGTA 661 AAGTCAGAAT GAATTCTTAT CCCTACCTAG CAGATAGACA TTATAGCCGG GAACAGCGTT 721 ACACTGATCA TGGGTCTGAC CATCATTACA GAGGAAAGAG AGATGATTTC TTCTATGAAG 781 ACAGGAATCA TGATGACTGG AGCCATGACT ATGATAACAG AAGAGACAGT AGTAGAGATG 841 GAAAATGGCG CTCATCTCGA CACTACCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 901 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 961 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAG TAAGCTAGAA 1021 CCTTCTCAAG CCAACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 1081 att