Construct: ORF TRCN0000477736
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012325.1_s317c1
- Derived from:
- ccsbBroadEn_09878
- DNA Barcode:
- TTAGAGAAAGGGAATACCTCACTG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- LRWD1 (222229)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477736
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 222229 | LRWD1 | leucine rich repeats and WD... | NM_152892.3 | 99.9% | 93.3% | 1815G>A |
| 2 | human | 222229 | LRWD1 | leucine rich repeats and WD... | NM_001317721.1 | 76.4% | 69.8% | 0_1ins456;1359G>A |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1878
- ORF length:
- 1812
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg ccccctctcg gcgcggctgc taatgcagcg cgggcgcccc aagagcgacc 121 ggctggggaa gatccggagt ctggacctgt caggattgga gctgctttcc gagcacctgg 181 accccaaact cctgtgccgc ctgacgcagc tgcaggagct tgacctgtct aacaaccacc 241 tggagacgct gccggacaac ctgggcctgt cccacctgcg tgtcctccgc tgcgccaaca 301 accagctggg ggatgttact gccttgtgcc agttccccaa gctcgaggaa ctcagcctgg 361 agggcaaccc cttcctgacg gtcaatgaca acctgaaagt ctcctttctc ctgcccacgc 421 tccgtaaggt caatggcaag gatgcgtcct caacttactc tcaggtggag aacctgaatc 481 gggagctgac cagcagggtc acagctcact gggagaagtt catggccaca ctgggtcctg 541 aagaggaggc tgagaaggcc caggcggact ttgtgaagtc ggctgtcagg gatgtccgct 601 acgggcccga gtccctcagc gagttcaccc agtggcgggt gcggatgatc tctgaggagc 661 tggtggccgc cagtaggacc caggtgcaaa aggctaacag cccagagaag cccccagaag 721 ctggagctgc ccacaagccc agggccagac tggcggcctt gaaacggcca gacgacgtcc 781 cactcagcct ctctcccagc aagcgggcgt gtgcctcccc gtcggcccag gtggagggca 841 gccctgtggc aggctccgat ggcagccagc ctgctgtgaa gctggagccc ctgcacttcc 901 tgcagtgcca cagcaagaac aacagccccc aggacctcga gacccagctg tgggcctgtg 961 ccttcgagcc ggcctgggag gagggggcca catcccagac cgtggccacg tgcggcgggg 1021 aggctgtgtg cgtaattgat tgccagacgg gcatcgtgct ccacaagtac aaggcacccg 1081 gcgaggagtt cttttctgtg gcctggaccg ctctgatggt ggtcacacag gctggccaca 1141 agaagcgctg gagtgtgctg gcggctgcag gcctacgggg cctggtccgg ctgctgcacg 1201 tgcgtgccgg cttctgctgc ggggtcatcc gagcccacaa gaaggccatc gccaccctgt 1261 gcttcagccc cgcccacgag acccatctct tcacggcctc ctatgacaag cggatcatcc 1321 tctgggacat cggggtgccc aaccaggact acgaattcca ggccagccag ctgctcacac 1381 tggacaccac ctctatcccc ctgcgcctct gccctgtcgc ctcctgcccg gacgcccgcc 1441 tgctggccgg ctgcgagggc ggctgctgct gctgggacgt gcggctggac cagccccaaa 1501 agaggagggt gtgtgaagtg gaattcgtct tctctgaggg cTCCGAGGCA TCTGGACGGA 1561 GAGTGGATGG GCTGGCATTT GTGAATGAGG ACATCGTGGC CTCCAAGGGG AGCGGCCTGG 1621 GCACCATCTG CCTGTGGAGC TGGAGGCAGA CGTGGGGGGG CCGGGGCAGC CAGTCCACGG 1681 TGGCAGTGGT GGTCCTGGCG CGGCTGCAAT GGTCGTCCAC CGAGTTGGCC TACTTCTCGC 1741 TCAGCGCCTG CCCTGATAAG GGGATTGTGC TCTGTGGGGA TGAGGAGGGC AACGTGTGGC 1801 TCTACGACGT CAGCAACATC CTGAAGCAGC CACCCCTGCT GCCGGCAGCC CTGCAGGCCC 1861 CCACACAGAT CCTGAAGTGA CCCCAGCCCT GGGCCCTTGG CCAGGTGGTG ACCAAGACCA 1921 TGGTGAACAC AGTGGTGGCC AATGCCTCCT TCACCTACCT CACCGCCCTG ACGGACTCCA 1981 ACATCGTAGC CATCTGGGGG AGGATGTACC CAACTTTCTT GTACAAAGTG GTTGATATCG 2041 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 2101 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GATTAGAGAA 2161 AGGGAATACC TCACTGACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 2221 aagatt