Construct: ORF TRCN0000477745
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015029.1_s317c1
- Derived from:
- ccsbBroadEn_10072
- DNA Barcode:
- GACCCGTATCGCCTCCAACACTAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NAT16 (375607)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477745
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 375607 | NAT16 | N-acetyltransferase 16 (put... | NM_001369694.1 | 99.9% | 99.7% | 188T>C |
| 2 | human | 375607 | NAT16 | N-acetyltransferase 16 (put... | NM_001369695.1 | 99.9% | 99.7% | 188T>C |
| 3 | human | 375607 | NAT16 | N-acetyltransferase 16 (put... | NM_198571.3 | 99.9% | 99.7% | 188T>C |
| 4 | human | 375607 | NAT16 | N-acetyltransferase 16 (put... | XM_024446759.1 | 56.7% | 50.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1176
- ORF length:
- 1107
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gaagctggaa gccagctgtg gcacagccac ctcagaggtc cctaagccgg 121 aaaagaagac tgcccgagat gcagagccaa gctctgaaac ccggccacag gaggtggagg 181 ccgagcccag gtcgggatcg gggcctgagg ctgaggccga gccattggac ttcgtggtgg 241 ccacggaacg ggagtctgag gaagtgctgg ccatctcggg gggcatctac ggcggcctgg 301 actaccttcc tagccgctac cacagctggc tccgggaccc cgaccgcacg gtggtgctgg 361 ccaagcgcaa cggaggcgtg atcgcgctgg agtcggtgaa cgtgatcgac gccggggaga 421 cggtgctggt ggaggggctg cgcgtggcgc cctgggagcg cgggaagggc gtggccgggc 481 tgctgcagcg cttctgctcg cagctggtca agagacagca cccgggggtc aaggtggcac 541 ggctcacccg ggacgaccag ctgggccccc gggagctgaa gaaataccgc ctaatcacca 601 agcagggcat ccttttggtc cgattcaacg cgtccgcgct gctggccggg ctgggcgcgc 661 ggctggcggc gctgcggacc tctggcacct tctcgccgct gcccaccgag gccgtgtccg 721 aggcaggcgg cgacgtggca cgcctcctgc tgtcaccctc cgtgcagcgc gacgtgcttc 781 caggcgggac catcatccag gactggcagc cctaccggcc tagcgaaagc aacctgcgcc 841 tgctggcggc caagggcctg gagtggcgcg tggacagccg cgcgcgcccg cgcgtgctca 901 cgctgtgcac gcgccccttc cccatcccgc acggagggga cggcacttgg cgctatctca 961 acatcgacgc cTTCGGTAGC GACGGCGCGC AGGTGCAGAG CCAGCTGCTG TGGCACCTGC 1021 AGCGCCAGGC CCCGCGCCTC GTTGGCCTCA ACGTCATGTG CCAGCTCTTC CTGGAGCCCC 1081 AGCTGTGGTC ACAGCTGGCT GACTTCTGCC AGGTCGGGCT GGGACTGGAG CTGGTGAAGG 1141 GTTATACTGA ACAGTACCTG CTGGAGGCCG ACATCTTGCC AACTTTCTTG TACAAAGTGG 1201 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 1261 CTAGCCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 1321 AGACCCGTAT CGCCTCCAAC ACTAAACGCG TTAAGTCgac aatcaacctc tggattacaa 1381 aatttgtgaa agatt