Construct: ORF TRCN0000477750
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015006.1_s317c1
- Derived from:
- ccsbBroadEn_08196
- DNA Barcode:
- TACAGGATTATCATGGATCCATTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HDDC2 (51020)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477750
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51020 | HDDC2 | HD domain containing 2 | NM_016063.3 | 99.8% | 99.5% | 392A>G |
| 2 | human | 51020 | HDDC2 | HD domain containing 2 | XM_024446450.1 | 75.8% | 75.4% | 0_1ins147;245A>G |
| 3 | human | 51020 | HDDC2 | HD domain containing 2 | XR_245538.4 | 44.2% | (many diffs) | |
| 4 | mouse | 69692 | Hddc2 | HD domain containing 2 | NM_027168.2 | 84.5% | 86.2% | (many diffs) |
| 5 | mouse | 69692 | Hddc2 | HD domain containing 2 | XM_006512836.3 | 53.1% | 53.3% | (many diffs) |
| 6 | mouse | 69692 | Hddc2 | HD domain containing 2 | XR_380073.3 | 41.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 681
- ORF length:
- 612
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcttcggtc tcctctgcga ccttctcggg ccacggggct cggtccctac 121 tgcagttcct gcggctggta gggcagctca agagagtccc acgaactggc tgggtataca 181 gaaatgtcca gaggccggag agcgtttcag atcacatgta ccggatggca gttatggcta 241 tggtgatcaa agatgaccgt cttaacaaag accgatgtgt acgcctagcc ctggttcatg 301 atatggcaga atgcatcgtt ggggacatag caccagcaga taacatcccc aaagaagaaa 361 aacataggcg agaagaggaa gctatgaagc agataaccca gctcctacca gaggacctca 421 gaaaggagct ctatgaactt tgggaagagt acgagacccg atcTAGTGCA GAAGCCAAAT 481 TTGTGAAGCA GCTAGACCAA TGTGAAATGA TTCTTCAAGC ATCTGAATAT GAAGACCTTG 541 AACACAAACC TGGGAGACTG CAAGACTTCT ATGATTCCAC AGCAGGAAAA TTCAATCACC 601 CTGAGATAGT CCAGCTTGTT TCTGAACTTG AGGCAGAAAG AAGCACTAAC ATAGCTGCAG 661 CTGCCAGTGA GCCACACTCC TTGCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 721 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 781 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGATACA GGATTATCAT 841 GGATCCATTA ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt