Construct: ORF TRCN0000477762
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011762.1_s317c1
- Derived from:
- ccsbBroadEn_06272
- DNA Barcode:
- ACAAGACATCAAGTACGGGCCTAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GHSR (2693)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477762
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2693 | GHSR | growth hormone secretagogue... | NM_198407.2 | 99.9% | 100% | 171C>T |
| 2 | human | 2693 | GHSR | growth hormone secretagogue... | NM_004122.2 | 77.1% | 71.7% | (many diffs) |
| 3 | mouse | 208188 | Ghsr | growth hormone secretagogue... | NM_177330.4 | 89.5% | 95.3% | (many diffs) |
| 4 | mouse | 208188 | Ghsr | growth hormone secretagogue... | XM_017319513.1 | 76.3% | 81.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1167
- ORF length:
- 1098
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gtggaacgcg acgcccagcg aagagccggg gttcaacctc acactggccg 121 acctggactg ggatgcttcc cccggcaacg actcgctggg cgacgagctg ctgcagctct 181 tccccgcgcc gctgctggcg ggcgtcacag ccacctgcgt ggcactcttc gtggtgggta 241 tcgctggcaa cctgctcacc atgctggtgg tgtcgcgctt ccgcgagctg cgcaccacca 301 ccaacctcta cctgtccagc atggccttct ccgatctgct catcttcctc tgcatgcccc 361 tggacctcgt tcgcctctgg cagtaccggc cctggaactt cggcgacctc ctctgcaaac 421 tcttccaatt cgtcagtgag agctgcacct acgccacggt gctcaccatc acagcgctga 481 gcgtcgagcg ctacttcgcc atctgcttcc cactccgggc caaggtggtg gtcaccaagg 541 ggcgggtgaa gctggtcatc ttcgtcatct gggccgtggc cttctgcagc gccgggccca 601 tcttcgtgct agtcggggtg gagcacgaga acggcaccga cccttgggac accaacgagt 661 gccgccccac cgagtttgcg gtgcgctctg gactgctcac ggtcatggtg tgggtgtcca 721 gcatcttctt cttccttcct gtcttctgtc tcacggtcct ctacagtctc atcggcagga 781 agctgtggcg gaggaggcgc ggcgatgctg tcgtgggtgc ctcgctcagg gaccagaacc 841 acaagcaaac cgtgaaaatg ctggctgtag tggtgtttgc cttcatcctc tgctggctcc 901 ccttccacgt agggcgatat ttattttcca aatcctttga gcctggctcc ttggagattg 961 ctcagatcag ccagtactgc aacctcgtgt cctttgtcct cttctacctc agtgctgcca 1021 tcaaccccat tctgtacaac atcatgtcca agaagtaccg ggtggcagtg ttcagacttc 1081 tgggattcga acccttctcc cagagaaagc tctccactct GAAAGATGAA AGTTCTCGGG 1141 CCTGGACAGA ATCTAGTATT AATACATTGC CAACTTTCTT GTACAAAGTG GTTGATATCG 1201 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 1261 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAACAAGACA 1321 TCAAGTACGG GCCTATACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 1381 aagatt