Construct: ORF TRCN0000477770
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014651.1_s317c1
- Derived from:
- ccsbBroadEn_00556
- DNA Barcode:
- CTCGTAGTGACCCAGATACCACTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FGF3 (2248)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477770
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2248 | FGF3 | fibroblast growth factor 3 | NM_005247.4 | 100% | 100% |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 786
- ORF length:
- 717
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gggcctaatc tggctgctac tgctcagcct gctggagccc ggctggcccg 121 cagcgggccc tggggcgcgg ttgcggcgcg atgcgggcgg ccgtggcggc gtctacgagc 181 accttggcgg ggcgccccgg cgccgcaagc tctactgcgc cacgaagtac cacctccagc 241 tgcacccgag cggccgcgtc aacggcagcc tggagaacag cgcctacagt attttggaga 301 taacggcagt ggaggtgggc attgtggcca tcaggggtct cttctccggg cggtacctgg 361 ccatgaacaa gaggggacga ctcTATGCTT CGGAGCACTA CAGCGCCGAG TGCGAGTTTG 421 TGGAGCGGAT CCACGAGCTG GGCTATAATA CGTATGCCTC CCGGCTGTAC CGGACGGTGT 481 CTAGTACGCC TGGGGCCCGC CGGCAGCCCA GCGCCGAGAG ACTGTGGTAC GTGTCTGTGA 541 ACGGCAAGGG CCGGCCCCGC AGGGGCTTCA AGACCCGCCG CACACAGAAG TCCTCCCTGT 601 TCCTGCCCCG CGTGCTGGAC CACAGGGACC ACGAGATGGT GCGGCAGCTA CAGAGTGGGC 661 TGCCCAGACC CCCTGGTAAG GGGGTCCAGC CCCGACGGCG GCGGCAGAAG CAGAGCCCGG 721 ATAACCTGGA GCCCTCTCAC GTTCAGGCTT CGAGACTGGG CTCCCAGCTG GAGGCCAGTG 781 CGCACTTGCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 841 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 901 CTTGGCTTTA TATATCTTGT GGAAAGGACG ACTCGTAGTG ACCCAGATAC CACTCACGCG 961 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt