Construct: ORF TRCN0000477775
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006337.1_s317c1
- Derived from:
- ccsbBroadEn_14493
- DNA Barcode:
- ATGCACGCGCACGTTTCAGTCACG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- VGLL3 (389136)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477775
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 389136 | VGLL3 | vestigial like family member 3 | NM_001320493.2 | 99.8% | 99.6% | 80C>T |
| 2 | human | 389136 | VGLL3 | vestigial like family member 3 | NM_016206.4 | 97% | 96% | (many diffs) |
| 3 | human | 389136 | VGLL3 | vestigial like family member 3 | XM_006713138.4 | 96.7% | 95.7% | (many diffs) |
| 4 | human | 389136 | VGLL3 | vestigial like family member 3 | NM_001320494.2 | 80.8% | 79.7% | (many diffs) |
| 5 | mouse | 73569 | Vgll3 | vestigial like family member 3 | XM_006523097.3 | 85.9% | 84.8% | (many diffs) |
| 6 | mouse | 73569 | Vgll3 | vestigial like family member 3 | NM_028572.1 | 85.6% | 84.4% | (many diffs) |
| 7 | mouse | 73569 | Vgll3 | vestigial like family member 3 | XM_006523098.2 | 72% | 71.7% | (many diffs) |
| 8 | mouse | 73569 | Vgll3 | vestigial like family member 3 | XM_006523099.3 | 70.9% | 69.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1029
- ORF length:
- 960
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gagttgtgcg gaggtgatgt atcaccccca gccttatgga gcgtcccagt 121 atctgcccaa ccccatggca gcgacaatct gccccacagc ctactatcag ccggcgcccc 181 aacctggcca gcagaagaag ttagcggtat tcagcaagat gcaggactct ctggaagtca 241 cccttcccag caaacaagag gaggaggatg aggaggagga ggaggaggag aaagaccagc 301 ctgccgagat ggagtacctt aactctcgct gtgtcctttt cacttatttc cagggagaca 361 ttgggtcagt agtggatgaa cacttctcaa gagctttggg ccaagccatc accctccatc 421 cagaatctgc catttcaaaa agcaagatgg ggctaacccc cctatggcga gacagctcag 481 ctctctcaag ccagcggaat agtttcccaa cttccttttg gaccagctct taccagcccc 541 cacctgcacc ttgtttgggg ggagttcatc ctgacttcca ggtcactgga ccccctggca 601 ccttttctgc agctgatccc agtccttggc cgggacacaa cctgcatcag actggcccag 661 cccctccccc tgctgtgtct gagtcctggc cttatccttt gacatctcag gtgagcccat 721 cctacagcca tatgcatgac gtgtacatgc ggcaccacca cCCTCATGCC CACATGCACC 781 ACCGCCACCG CCACCATCAT CACCATCACC ACCCTCCTGC TGGCTCTGCC CTGGATCCAT 841 CCTATGGGCC TCTGCTGATG CCTTCAGTGC ATGCGGCCAG GATTCCTGCT CCCCAGTGTG 901 ACATCACAAA GACAGAACCA ACTACAGTCA CCTCTGCTAC CTCAGCATGG GCTGGAGCCT 961 TTCATGGAAC AGTAGACATA GTGCCCAGCG TGGGATTCGA TACAGGCTGG AGTGCAATGG 1021 CGAGATCTTT GCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 1081 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 1141 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAATGCAC GCGCACGTTT CAGTCACGAC 1201 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt