Construct: ORF TRCN0000477780
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011564.1_s317c1
- Derived from:
- ccsbBroadEn_13594
- DNA Barcode:
- ACAACTCCCAGCCCAATATCGACC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NAT8L (339983)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477780
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 339983 | NAT8L | N-acetyltransferase 8 like | NM_178557.4 | 44.3% | 44.3% | 1_504del |
| 2 | mouse | 269642 | Nat8l | N-acetyltransferase 8-like | NM_001001985.3 | 40.4% | 43.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 471
- ORF length:
- 402
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcggacatc gagcagtact acatgaagcc gcccggctcc TGCTTCTGGG 121 TGGCCGTGCT GGATGGCAAC GTGGTGGGCA TTGTGGCTGC ACGGGCCCAC GAGGAGGACA 181 ACACGGTGGA GCTGCTGCGG ATGTCTGTGG ACTCACGTTT CCGAGGCAAG GGCATCGCCA 241 AGGCGCTGGG CCGGAAGGTG CTGGAGTTCG CCGTGGTGCA CAACTACTCC GCGGTGGTGC 301 TGGGCACGAC GGCCGTCAAG GTGGCCGCCC ACAAGCTCTA CGAGTCGCTG GGCTTCAGAC 361 ACATGGGCGC CAGTGACCAC TACGTGCTGC CGGGCATGAC CCTCTCGCTG GCTGAGCGCC 421 TCTTCTTCCA GGTCCGCTAC CACCGCTACC GCCTGCAGCT GCGCGAGGAG TTGCCAACTT 481 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 541 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 601 CTTGTGGAAA GGACGAACAA CTCCCAGCCC AATATCGACC ACGCGTTAAG TCgacaatca 661 acctctggat tacaaaattt gtgaaagatt